PUBLICATION
Asymmetric localization of Numb:EGFP in dividing neuroepithelial cells during neurulation in Danio rerio
- Authors
- Reugels, A.M., Boggetti, B., Scheer, N., and Campos-Ortega, J.A.
- ID
- ZDB-PUB-060424-8
- Date
- 2006
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 235(4): 934-948 (Journal)
- Registered Authors
- Boggetti, Barbara, Campos-Ortega, Jose, Reugels, Alexander, Scheer, Nico
- Keywords
- Danio rerio, zebrafish, neurulation, neuroepithelial polarity, spindle orientation, asymmetric localization, Numb, Numblike, PTB domain, PRR
- MeSH Terms
-
- Molecular Sequence Data
- Phylogeny
- Cell Membrane/metabolism
- Gene Deletion
- Animals
- Green Fluorescent Proteins/metabolism*
- Spindle Apparatus/physiology
- Mutagenesis, Insertional
- Neuroepithelial Cells/cytology
- Neuroepithelial Cells/physiology*
- Embryo, Nonmammalian
- Conserved Sequence
- Recombinant Fusion Proteins/chemistry
- Recombinant Fusion Proteins/metabolism
- Cloning, Molecular
- Drosophila Proteins/chemistry
- Drosophila Proteins/genetics
- Drosophila Proteins/metabolism*
- Juvenile Hormones/chemistry
- Juvenile Hormones/genetics
- Juvenile Hormones/metabolism*
- Nerve Tissue Proteins/chemistry
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism
- Microscopy, Confocal
- Protein Isoforms/chemistry
- Protein Isoforms/genetics
- Cell Polarity*
- Zebrafish/embryology*
- Zebrafish/genetics
- Protein Structure, Tertiary
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Homology, Amino Acid
- Membrane Proteins
- Microscopy, Video
- Introns
- Amino Acid Sequence
- PubMed
- 16493689 Full text @ Dev. Dyn.
Citation
Reugels, A.M., Boggetti, B., Scheer, N., and Campos-Ortega, J.A. (2006) Asymmetric localization of Numb:EGFP in dividing neuroepithelial cells during neurulation in Danio rerio. Developmental Dynamics : an official publication of the American Association of Anatomists. 235(4):934-948.
Abstract
In the neural plate and tube of the zebrafish embryo, cells divide with their mitotic spindles oriented parallel to the plane of the neuroepithelium, whilst in the neural keel and rod, the spindle is oriented perpendicular to it. This change is achieved by a 90 degrees rotation of the mitotic spindle. We cloned zebrafish homologues of the gene for the Drosophila cell fate determinant Numb, and analyzed the localization of EGFP fusion proteins in vivo in dividing neuroepithelial cells during neurulation. Whereas Numb isoform 3 and the related protein Numblike are localized in the cytoplasm, Numb isoform 1 is localized to the cell membrane. Time-lapse analyses showed that Numb 1 is distributed uniformly around the cell cortex in dividing cells during plate and keel stages, but becomes localized at the basolateral membrane of some dividing cells during the transition from neural rod to tube. Using in vitro mutagenesis and Numb:EGFP deletion constructs, we showed that the first 196 amino acids of Numb are sufficient for this localization. Furthermore, we found that an 11-amino acid insertion in the PTB domain is essential for localization to the cortex, whereas amino acids 2-12 mediate the basolateral localization in the neural tube stage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping