PUBLICATION

Zebrafish id2 developmental expression pattern contains evolutionary conserved and species-specific characteristics

Authors
Chong, S.W., Nguyen, T.T., Chu, L.T., Jiang, Y.J., Korzh, V.
ID
ZDB-PUB-051031-4
Date
2005
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   234(4): 1055-1063 (Journal)
Registered Authors
Chong, Shang Wei, Chu, Lee Thean, Jiang, Yun-Jin, Korzh, Vladimir, Nguyen, Thi Thu Hang
Keywords
CNS, amacrine cells, biphasic expression, corpuscles of Stannius, mind bomb, deadly seven, beamter
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain/metabolism*
  • Cluster Analysis
  • Cryoultramicrotomy
  • DNA Primers
  • DNA, Complementary/genetics
  • Gene Expression Regulation, Developmental*
  • Inhibitor of Differentiation Protein 2/genetics
  • Inhibitor of Differentiation Protein 2/metabolism*
  • Molecular Sequence Data
  • Receptors, Notch/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Signal Transduction/genetics
  • Signal Transduction/physiology*
  • Species Specificity
  • Zebrafish/embryology*
PubMed
16252281 Full text @ Dev. Dyn.
Abstract
The inhibitor of differentiation or inhibitor of DNA binding (Id) family are members of the helix-loop-helix (HLH) group of transcription factors that play important roles in cell proliferation, differentiation, cell cycle control, and apoptosis. They modulate the formation of active class A-class B basic HLH (bHLH) complexes. Ids lack the amino-terminal associated basic region necessary for DNA binding, thus sequestering the class A factors, inhibiting the formation of active class A-class B heterodimers and, therefore, are considered to act as dominant-negative regulators of differentiation pathways. We isolated zebrafish id2, and its expression during development was characterized. id2, in addition to regions of expression detected in Xenopus and mice, is also expressed in the tegmentum; midbrain-hindbrain boundary; cerebellum; rhombomeres 2,3,4,6; notochord; and corpuscles of Stannius. Furthermore, we show that expression of id2 is repressed in mind bomb mutants, suggesting a role of Notch upstream of Id2. Developmental Dynamics, 2005. (c) 2005 Wiley-Liss, Inc.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping