PUBLICATION

Eomesodermin is a localized maternal determinant required for endoderm induction in zebrafish

Authors
Bjornson, C.R., Griffin, K.J., Farr, G.H. 3rd, Terashima, A., Himeda, C., Kikuchi, Y., and Kimelman, D.
ID
ZDB-PUB-051005-5
Date
2005
Source
Developmental Cell   9(4): 523-533 (Journal)
Registered Authors
Bjornson, Chris, Griffin, Kevin, Kikuchi, Yutaka, Kimelman, David
Keywords
none
MeSH Terms
  • Protein Binding
  • Animals
  • SOX Transcription Factors
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Transcription, Genetic
  • Transforming Growth Factor beta/genetics
  • Transforming Growth Factor beta/metabolism
  • Gene Expression Regulation, Developmental
  • In Situ Hybridization
  • Oligoribonucleotides, Antisense/genetics
  • Oligoribonucleotides, Antisense/metabolism
  • Embryonic Induction*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Nodal Protein
  • Endoderm/cytology
  • Endoderm/physiology*
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology*
  • T-Box Domain Proteins/genetics
  • T-Box Domain Proteins/metabolism*
  • GATA5 Transcription Factor/genetics
  • GATA5 Transcription Factor/metabolism
  • High Mobility Group Proteins/genetics
  • High Mobility Group Proteins/metabolism*
  • Promoter Regions, Genetic
PubMed
16198294 Full text @ Dev. Cell
Abstract
In zebrafish, endoderm induction occurs in marginal blastomeres and requires Casanova (Cas), the first endoderm-specific factor expressed in the embryo. Whereas the transcription factors Gata5 and Bon are necessary and sufficient for cas expression in marginal blastomeres, Bon and Gata5 are unable to induce cas in animal pole cells, suggesting that cas expression requires an additional, unidentified factor(s). Here, we show that cas expression depends upon the T box transcription factor Eomesodermin (Eomes), a maternal determinant that is localized to marginal blastomeres. Eomes synergizes potently with Bon and Gata5 to induce cas, even in animal pole blastomeres. We show that Eomes is required for endogenous endoderm induction, acting via an essential binding site in the cas promoter. Direct physical interactions between Eomes, Bon, and Gata5 suggest that Eomes promotes endoderm induction in marginal blastomeres by facilitating the assembly of a transcriptional activating complex on the cas promoter.
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