PUBLICATION
Zebrafish R-cadherin (Cdh4) controls visual system development and differentiation
- Authors
- Babb, S.G., Kotradi, S.M., Shah, B., Chiappini-Williamson, C., Bell, L.N., Schmeiser, G., Chen, E., Liu, Q., and Marrs, J.A.
- ID
- ZDB-PUB-050603-2
- Date
- 2005
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 233(3): 930-945 (Journal)
- Registered Authors
- Clendenon, Sherry, Liu, Qin, Marrs, James A.
- Keywords
- R-cadherin; zebrafish; visual system development; axonogenesis; antisense oligonucleotide
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Apoptosis/genetics
- Cadherins/genetics
- Cadherins/metabolism*
- Cell Differentiation*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental
- Mutation/genetics
- Neurons/cytology
- Neurons/metabolism
- Phenotype
- Retina/cytology*
- Retina/embryology*
- Retina/metabolism
- Vision, Ocular/physiology*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 15918170 Full text @ Dev. Dyn.
Citation
Babb, S.G., Kotradi, S.M., Shah, B., Chiappini-Williamson, C., Bell, L.N., Schmeiser, G., Chen, E., Liu, Q., and Marrs, J.A. (2005) Zebrafish R-cadherin (Cdh4) controls visual system development and differentiation. Developmental Dynamics : an official publication of the American Association of Anatomists. 233(3):930-945.
Abstract
In zebrafish, R-cadherin (cadherin-4 or Cdh4) is expressed in the retina and in retinorecipient brain regions, suggesting that Cdh4 functions during visual system development. Cdh4 function was examined during retinogenesis and retinal axon outgrowth using antisense morpholino oligonucleotides and mutant Cdh4 construct expression. In knockdowns, Cdh4 was reduced or absent, eyes were small, and retinae lacked discrete laminae. Increased cell death produced the small eye phenotype. Zn5-, Pax6-, and zpr-1-positive cells were reduced or absent in knockdown retinas but, when present, were in the correct laminae. Cdh4 knockdowns had sparse or absent retinal ganglion cell axons. When present, axons projected contralaterally but lacked fine branching and failed to reach the tectum or arborize the entire tectum. Mutant Cdh4 construct expression during retinal ganglion cell differentiation reduced or ablated neurite formation. Cdh4 is necessary for neural retina survival and differentiation, and required for normal retinotectal projection formation and tectal arborization. Developmental Dynamics, 2005. (c) 2005 Wiley-Liss, Inc.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping