PUBLICATION

Evolutionarily conserved non-AUG translation initiation in NAT1/p97/DAP5 (EIF4G2)

Authors

Takahashi, K., Maruyama, M., Tokuzawa, Y., Murakami, M., Oda, Y., Yoshikane, N., Makabe, K.W., Ichisaka, T., and Yamanaka, S.

ID

ZDB-PUB-050221-9

Date

2005

Source

Genomics 85(3): 360-371 (Journal)

Registered Authors

Keywords

none

MeSH Terms

Amino Acid Sequence
Base Sequence
Blotting, Western
Drosophila Proteins/genetics*
Eukaryotic Initiation Factor-4G/chemistry
Eukaryotic Initiation Factor-4G/genetics*
Evolution, Molecular*
Molecular Sequence Data
Mutagenesis, Site-Directed
Protein Biosynthesis*
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Two-Hybrid System Techniques

PubMed

15718103 Full text @ Genomics

Abstract

Only a few cases of exclusive translation initiation at non-AUG codons have been reported. We recently demonstrated that mammalian NAT1 mRNA, encoded by EIF4G2, uses GUG as its only translation initiation codon. In this study, we identified NAT1 orthologs from chicken, Xenopus, and zebrafish and found that in all species, the GUG codon also serves as the initiation codon. In all species, the GUG codon fulfilled the reported requirements for non-AUG initiation: an optimal Kozak motif and a downstream hairpin structure. Site-directed mutagenesis showed that nucleotides at positions -3 and +4 are critical for the GUG-mediated translation initiation in vitro. We found that NAT1 orthologs in Drosophila melanogaster and Halocynthia roretzi also use non-AUG start codons, demonstrating evolutionary conservation of the noncanonical translation initiation.

Genes / Markers

Figures

Expression

Phenotype

Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Fish

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Takahashi et al., 2005 ZDB-PUB-050221-9 PMID:15718103

Evolutionarily conserved non-AUG translation initiation in NAT1/p97/DAP5 (EIF4G2)

Takahashi et al., 2005

ZDB-PUB-050221-9
PMID:15718103