PUBLICATION
Zebrafish Polycomb group gene ph2alpha is required for epiboly and tailbud formation acting downstream of FGF signaling
- Authors
- Komoike, Y., Kawamura, A., Shindo, N., Sato, C., Satoh, J., Shiurba, R., and Higashinakagawa, T.
- ID
- ZDB-PUB-050215-4
- Date
- 2005
- Source
- Biochemical and Biophysical Research Communications 328(4): 858-866 (Journal)
- Registered Authors
- Kawamura, Akinori
- Keywords
- Zebrafish; ph2alpha; PcG; Epiboly; Tailbud; FGF signaling
- MeSH Terms
-
- Animals
- Carrier Proteins/genetics
- Carrier Proteins/metabolism*
- Fibroblast Growth Factors/metabolism*
- Gene Expression Regulation, Developmental/physiology*
- Gene Silencing
- Limb Buds
- Polycomb-Group Proteins
- Repressor Proteins/genetics
- Repressor Proteins/metabolism*
- Signal Transduction/physiology
- Tissue Distribution
- Zebrafish/anatomy & histology
- Zebrafish/embryology*
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 15707958 Full text @ Biochem. Biophys. Res. Commun.
- CTD
- 15707958
Citation
Komoike, Y., Kawamura, A., Shindo, N., Sato, C., Satoh, J., Shiurba, R., and Higashinakagawa, T. (2005) Zebrafish Polycomb group gene ph2alpha is required for epiboly and tailbud formation acting downstream of FGF signaling. Biochemical and Biophysical Research Communications. 328(4):858-866.
Abstract
We analyzed Polycomb group gene ph2alpha functionally in zebrafish embryos by a gene knock-down procedure using morpholino antisense oligos. Inhibition of ph2alpha message translation resulted in abnormal epibolic movements as well as a thick tailbud or incomplete covering of the yolk plug. At the 24hpf stage, morphants had short trunks and tails, phenotypes similar to those with disturbances in FGF signaling. Accordingly, we looked at the effects of ph2alpha expression upstream and downstream of the FGF pathway. Treatment with SU5402, an inhibitor of Fgfrs, or injection of dominant-negative Fgfr1 DNA markedly reduced ph2alpha expression in the tailbud. In addition, cells expressing mRNAs for no tail, spadetail, myoD, and papc, which are involved in FGF-related development of posterior mesoderm, were distributed abnormally. Collectively, the data argue that ph2alpha is required for epiboly and tailbud formation, acting downstream of the FGF signaling pathway.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping