PUBLICATION

Interplay of pu.1 and gata1 determines myelo-erythroid progenitor cell fate in zebrafish

Authors
Rhodes, J., Hagen, A., Hsu, K., Deng, M., Liu, T.X., Look, A.T., and Kanki, J.P.
ID
ZDB-PUB-050104-3
Date
2005
Source
Developmental Cell   8(1): 97-108 (Journal)
Registered Authors
Hsu, Karl, Kanki, John, Liu, Ting Xi, Look, A. Thomas, Rhodes, Jennifer
Keywords
none
MeSH Terms
  • Cell Differentiation/physiology
  • Microinjections/methods
  • Flow Cytometry/methods
  • Models, Biological
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • GATA1 Transcription Factor
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins/physiology*
  • Erythroid-Specific DNA-Binding Factors
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/physiology*
  • Erythroid Precursor Cells/physiology*
  • RNA, Messenger/biosynthesis
  • Myeloid Progenitor Cells/physiology*
  • Animals
  • Animals, Genetically Modified
  • Genotype
  • Transcription Factors/genetics
  • Transcription Factors/physiology*
  • Cell Movement/physiology
  • Zebrafish/embryology
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Embryonic Induction
  • Reverse Transcriptase Polymerase Chain Reaction/methods
  • Trans-Activators/genetics
  • Trans-Activators/physiology*
  • Transplantation/methods
  • Gene Expression Regulation, Developmental/physiology
  • Hematopoiesis/physiology
  • In Situ Hybridization/methods
PubMed
15621533 Full text @ Dev. Cell
Abstract
The zebrafish is a powerful model system for investigating embryonic vertebrate hematopoiesis, allowing for the critical in vivo analysis of cell lineage determination. In this study, we identify zebrafish myeloerythroid progenitor cells (MPCs) that are likely to represent the functional equivalent of mammalian common myeloid progenitors. Utilizing transgenic pu.1-GFP fish, real-time MPC differentiation was correlated with dynamic changes in cell motility, morphology, and gene expression. Unlike mammalian hematopoiesis, embryonic zebrafish myelopoiesis and erythropoiesis occur in anatomically separate locations. Gene knockdown experiments and transplantation assays demonstrated the reciprocal negative regulation of pu.1 and gata1 and their non-cell-autonomous regulation that determines myeloid versus erythroid MPC fate in the distinct blood-forming regions. Furthermore, forced expression of pu.1 in the bloodless mutant cloche resulted in myelopoietic rescue, providing intriguing evidence that this gene can function in the absence of some stem cell genes, such as scl, in governing myelopoiesis.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping