PUBLICATION
Critical roles of CD146 in zebrafish vascular development
- Authors
- Chan, B., Sinha, S., Cho, D., Ramchandran, R., and Sukhatme, V.P.
- ID
- ZDB-PUB-041208-4
- Date
- 2005
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 232(1): 232-244 (Journal)
- Registered Authors
- Ramchandran, Ramani, Sinha, Soniya, Sukhatme, Vikas
- Keywords
- CD146, P1H12, Mel-CAM, zebrafish vascular development, morpholino, fluorescence microangiography
- MeSH Terms
-
- Amino Acid Sequence
- Angiography
- Animals
- Antigens, CD/metabolism
- Antigens, CD/physiology*
- Base Sequence
- Blood Vessels/embryology*
- CD146 Antigen
- Cloning, Molecular
- DNA, Complementary/metabolism
- Endothelium, Vascular/embryology*
- Gene Expression Regulation, Developmental*
- In Situ Hybridization
- Microscopy, Fluorescence
- Molecular Sequence Data
- Neural Cell Adhesion Molecules/metabolism
- Neural Cell Adhesion Molecules/physiology*
- Oligonucleotides/chemistry
- Protein Structure, Tertiary
- RNA, Messenger/metabolism
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Homology, Amino Acid
- Time Factors
- Zebrafish
- PubMed
- 15580611 Full text @ Dev. Dyn.
Citation
Chan, B., Sinha, S., Cho, D., Ramchandran, R., and Sukhatme, V.P. (2005) Critical roles of CD146 in zebrafish vascular development. Developmental Dynamics : an official publication of the American Association of Anatomists. 232(1):232-244.
Abstract
In this report, we use zebrafish as a model system to understand the importance of CD146 in vascular development. Endothelial-specific expression of CD146 was verified by whole-mount in situ hybridization. Suppression of CD146 protein expression by antisense morpholino oligonucleotides (MO) resulted in poorly developed intersomitic vessels (ISVs). In CD146 morphants, we observed a lack of blood flow through the ISV region, despite that fluorescence microangiography showed that the ISVs were present. This finding suggests that the lumens of the developing ISVs may be too narrow for proper circulation. Additionally, remodeling of the caudal vein plexus into functional vascular tubes appeared to be affected. Suppression of CD146 protein expression resulted in a circulation shunt that caused the circulation to by-pass part of the caudal artery/vein system. The same vascular defects were recapitulated by using a second morpholino oligonucleotide. This morphant expressed a truncated CD146 protein with amino acids V32 to T57 at the N terminus deleted in an in-frame manner. This region, therefore, is likely to contain elements critical for CD146 function. This study provides the first in vivo functional assessment of CD146 in embryonic development by showing that knockdown of CD146 protein expression severely hinders vascular development in zebrafish. Developmental Dynamics 232:000-000, 2005. (c) 2004 Wiley-Liss, Inc.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping