PUBLICATION
Zebrafish heparin-binding neurotrophic factor enhances neurite outgrowth during its development
- Authors
- Chang, M.H., Huang, C.J., Hwang, S.P., Lu, I.C., Lin, C.M., Kuo, T.F., and Chou, C.M.
- ID
- ZDB-PUB-040914-3
- Date
- 2004
- Source
- Biochemical and Biophysical Research Communications 321(2): 502-509 (Journal)
- Registered Authors
- Huang, Chang-Jen, Hwang, Sheng-Ping L.
- Keywords
- Heparin-binding neurotrophic factor; Neurite outgrowth; PC12 cells; Zebrafish; Neuron-specific promoter
- MeSH Terms
-
- Amino Acid Sequence
- Embryo, Nonmammalian
- Humans
- DNA, Complementary/genetics
- Rats
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Cell Size/drug effects
- Reverse Transcriptase Polymerase Chain Reaction
- Cloning, Molecular
- Sequence Alignment
- Gene Expression Regulation, Developmental
- Neurites/drug effects
- Neurites/metabolism*
- PC12 Cells
- Cytokines/chemistry
- Cytokines/genetics*
- Cytokines/metabolism*
- Cytokines/pharmacology
- Zebrafish/embryology*
- Zebrafish/genetics*
- Carrier Proteins/chemistry
- Carrier Proteins/genetics*
- Carrier Proteins/metabolism*
- Carrier Proteins/pharmacology
- Gene Expression Profiling
- Embryo, Mammalian/cytology
- Embryo, Mammalian/drug effects
- Embryo, Mammalian/metabolism
- Molecular Sequence Data
- Animals
- PubMed
- 15358204 Full text @ Biochem. Biophys. Res. Commun.
Citation
Chang, M.H., Huang, C.J., Hwang, S.P., Lu, I.C., Lin, C.M., Kuo, T.F., and Chou, C.M. (2004) Zebrafish heparin-binding neurotrophic factor enhances neurite outgrowth during its development. Biochemical and Biophysical Research Communications. 321(2):502-509.
Abstract
Heparin-binding neurotrophic factor (HBNF) is a secreted heparin-binding protein containing highly basic and cysteine-rich amino acid residues. In this study, we cloned the full-length HBNF cDNA from zebrafish and determined its genomic structure by bioinformatics analysis. Zebrafish HBNF gene is composed of five exons and four introns spanning approximately 82kb. RT-PCR analysis revealed that zebrafish HBNF transcript was highly expressed in adult brain and intestine tissues while less in other tissues. During embryogenesis, zebrafish HBNF transcript was observed to be moderately expressed at earlier stages with a gradual decline. Higher expression level was observed after hatching and maintaining this level into adulthood. The overall amino acid sequence of zebrafish HBNF shows 60% identity to human HBNF, but with approximately 40% identity to other midkine proteins. Like mammalian homolog, zebrafish HBNF could induce significant neurite outgrowth in PC12 cells without NGF stimulation. In addition, zebrafish HBNF was able to enhance extensive neurite outgrowth in zebrafish embryos during embryogenesis. In summary, a feasible in vivo assay for neurite outgrowth was established in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping