PUBLICATION
Molecular and Functional Characterization of Sex Hormone Binding Globulin in Zebrafish
- Authors
- Miguel-Queralt, S., Knowlton, M., Avvakumov, G.V., Al-Nouno, R., Kelly, G.M., and Hammond, G.L.
- ID
- ZDB-PUB-040804-7
- Date
- 2004
- Source
- Endocrinology 145(11): 5221-5320 (Journal)
- Registered Authors
- Hammond, Geoff, Kelly, Greg, Knowlton, Michelle
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- CHO Cells
- Cloning, Molecular
- Cricetinae
- Gene Expression Regulation, Developmental
- Immunohistochemistry
- Intestines/physiology
- Liver/physiology
- Male
- Molecular Sequence Data
- RNA, Messenger/analysis
- Sex Hormone-Binding Globulin/genetics*
- Sex Hormone-Binding Globulin/metabolism*
- Testis/physiology
- Zebrafish/genetics*
- PubMed
- 15284198 Full text @ Endocrinology
Citation
Miguel-Queralt, S., Knowlton, M., Avvakumov, G.V., Al-Nouno, R., Kelly, G.M., and Hammond, G.L. (2004) Molecular and Functional Characterization of Sex Hormone Binding Globulin in Zebrafish. Endocrinology. 145(11):5221-5320.
Abstract
Sex hormone-binding globulin (SHBG) transports androgens and estrogens in the blood of vertebrates including fish. Orthologues of SHBG in fish are poorly defined and we have now obtained a zebrafish SHBG cDNA and characterized the zebrafish SHBG gene and protein through molecular biological, biochemical and informatics approaches. Amino-terminal analysis of zebrafish SHBG indicated that its deduced precursor sequence includes a 25-residue secretion polypeptide, and exhibits 22-27% homology with mammalian SHBG sequences, and 41% with a deduced fugufish SHBG sequence. The 356 residue mature zebrafish SHBG (39,243 Da) sequence comprises a tandem repeat of laminin G-like domains typical of SHBG sequences; contains three N-glycosylation sites, and exists as a 105,000 +/- 8,700 Da homodimer. Zebrafish SHBG exhibits a high affinity and specificity for sex steroids. A reverse-transcriptase PCR indicated that SHBG mRNA first appears in zebrafish larva, and SHBG mRNA was localized within the liver and gut at this stage of development by whole mount in situ hybridization. In adult fish, SHBG mRNA was found in liver, testis and gut. In the liver, immunoreactive SHBG was present in hepatocytes and concentrated in intra-hepatic bile duct cells, while in the testis it was confined to cells surrounding the seminiferous tubule cysts. In the intestine, immunoreactive SHBG was present in the stroma and epithelial cells of the villous projections and the surrounding muscle. The production and presence of SHBG in the gut of developing and adult zebrafish suggests a novel role for this protein in regulating sex steroid action at this site.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping