PUBLICATION
Zebrafish cellular nucleic acid-binding protein: gene structure and developmental behaviour
- Authors
- Armas, P., Cachero, S., Lombardo, V.A., Weiner, A., Allende, M.L., and Calcaterra, N.B.
- ID
- ZDB-PUB-040803-3
- Date
- 2004
- Source
- Gene 337: 151-161 (Journal)
- Registered Authors
- Allende, Miguel L., Calcaterra, Nora
- Keywords
- CCHC-motif, Early embryogenesis, Single-stranded nucleic acid-binding protein, Developmental pattern expression, Whole-mount immunohistochemistry
- MeSH Terms
-
- RNA-Binding Proteins/genetics*
- RNA-Binding Proteins/metabolism
- DNA, Complementary/chemistry
- DNA, Complementary/genetics
- Genes/genetics
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/growth & development
- Male
- Molecular Sequence Data
- Female
- Ovary/embryology
- Ovary/growth & development
- Ovary/metabolism
- Cloning, Molecular
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Embryo, Nonmammalian/metabolism
- Embryonic Development
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Animals
- Protein Isoforms/genetics
- Protein Isoforms/metabolism
- Protein Binding
- RNA Probes/genetics
- RNA Probes/metabolism
- In Situ Hybridization
- Immunohistochemistry
- Amino Acid Sequence
- Alternative Splicing
- Sequence Analysis, DNA
- Gene Expression Regulation, Developmental
- Sequence Alignment
- PubMed
- 15276211 Full text @ Gene
Citation
Armas, P., Cachero, S., Lombardo, V.A., Weiner, A., Allende, M.L., and Calcaterra, N.B. (2004) Zebrafish cellular nucleic acid-binding protein: gene structure and developmental behaviour. Gene. 337:151-161.
Abstract
Here we analyse the structural organisation and expression of the zebrafish cellular nucleic acid-binding protein (zCNBP) gene and protein. The gene is organised in five exons and four introns. A noteworthy feature of the gene is the absence of a predicted promoter region. The coding region encodes a 163-amino acid polypeptide with the highly conserved general structural organisation of seven CCHC Zn knuckle domains and an RGG box between the first and the second Zn knuckles. Although theoretical alternative splicing is possible, only one form of zCNBP is actually detected. This form is able to bind to single-stranded DNA and RNA probes in vitro. The analysis of zCNBP developmental expression shows a high amount of CNBP-mRNA in ovary and during the first developmental stages. CNBP-mRNA levels decrease while early development progresses until the midblastula transition (MBT) stage and increases again thereafter. The protein is localised in the cytoplasm of blastomeres whereas it is mainly nuclear in developmental stages after the MBT. These findings suggest that CNBP is a strikingly conserved single-stranded nucleic acid-binding protein which might interact with maternal mRNA during its storage in the embryo cell cytoplasm. It becomes nuclear once MBT takes place possibly in order to modulate zygotic transcription and/or to associate with newly synthesised transcripts.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping