PUBLICATION

Comparative analysis of the PDCD2-TBP-PSMB1 region in vertebrates

Authors
Trachtulec, Z., Vlcek, C., Mihola, O., and Forejt, J.
ID
ZDB-PUB-040616-1
Date
2004
Source
Gene   335: 151-157 (Journal)
Registered Authors
Keywords
Conserved synteny, Danio rerio, Fugu rubripes, Gallus gallus, Tetraodon nigroviridis
MeSH Terms
  • Animals
  • Avian Proteins/genetics
  • Chickens/genetics
  • Chromosome Mapping
  • Cosmids/genetics
  • Cysteine Endopeptidases/genetics*
  • DNA/chemistry
  • DNA/genetics
  • Molecular Sequence Data
  • Nuclear Matrix/metabolism
  • Polymorphism, Genetic
  • Proteins/genetics*
  • Replication Origin/genetics
  • Sequence Analysis, DNA
  • Synteny
  • TATA-Box Binding Protein/genetics*
  • Takifugu/genetics
  • Tetraodontiformes/genetics
  • Vertebrates/genetics*
  • Zebrafish/genetics
PubMed
15194198 Full text @ Gene
Abstract
Three orthologous genes encoding programmed cell death 2 (PDCD2), TATA-binding protein (TBP), and proteasomal subunit C5 (PSMB1) proteins have been shown previously to be nonrandomly distributed in both mammalian and invertebrate genomes. Here we analyze a conserved synteny of the PDCD2, TBP, and PSMB1 orthologs in four nonmammalian vertebrates. Homologous genes of the chicken, zebrafish, fugu, and Tetraodon nigroviridis were identified. A chicken cosmid harboring the orthologs of these three genes was completely sequenced. The fish genes were analyzed in silico. In all seven vertebrates thus far investigated, the PDCD2 and TBP genes are located tail-to-tail. In six tested species but the zebrafish, the PSMB1 gene mapped head-to-head or in the close vicinity to the TBP, but even in the zebrafish, all three genes were syntenic. In contrast, a three times reused synteny breakpoint in the 5'-region from PDCD2 was detected. A comparative analysis revealed the distribution of putative matrix-attached regions (MARs), which may affect the synteny conservation.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping