PUBLICATION
Two zebrafish Notch-dependent hairy/Enhancer-of-split-related genes, her6 and her4, are required to maintain the coordination of cyclic gene expression in the presomitic mesoderm
- Authors
- Pasini, A., Jiang, Y.J., and Wilkinson, D.G.
- ID
- ZDB-PUB-040319-9
- Date
- 2004
- Source
- Development (Cambridge, England) 131(7): 1529-1541 (Journal)
- Registered Authors
- Jiang, Yun-Jin, Wilkinson, David
- Keywords
- none
- MeSH Terms
-
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Somites/physiology
- Helix-Loop-Helix Motifs
- Receptors, Notch
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Intracellular Signaling Peptides and Proteins
- Zebrafish/embryology*
- Zebrafish/genetics
- Animals
- Oligonucleotides, Antisense/genetics
- Oligonucleotides, Antisense/metabolism
- Biological Clocks/physiology
- Morphogenesis
- Body Patterning*
- Signal Transduction/physiology
- In Situ Hybridization
- Genes, Reporter
- Mesoderm/cytology
- Mesoderm/physiology*
- Basic Helix-Loop-Helix Transcription Factors
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism
- Trans-Activators/metabolism*
- Membrane Proteins/genetics
- Membrane Proteins/metabolism*
- Gene Expression Regulation, Developmental*
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism*
- PubMed
- 15023930 Full text @ Development
Citation
Pasini, A., Jiang, Y.J., and Wilkinson, D.G. (2004) Two zebrafish Notch-dependent hairy/Enhancer-of-split-related genes, her6 and her4, are required to maintain the coordination of cyclic gene expression in the presomitic mesoderm. Development (Cambridge, England). 131(7):1529-1541.
Abstract
Alterations of the Delta/Notch signalling pathway cause multiple morphogenetic abnormalities in somitogenesis, including defects in intersomitic boundary formation and failure in maintenance of somite regularity. Notch signalling has been implicated in establishing the anteroposterior polarity within maturing somites and in regulating the activity of a molecular segmentation clock operating in the presomitic mesoderm. The pleiotropy of Notch signalling obscures the roles of this pathway in different steps of somitogenesis. One possibility is that distinct Notch effectors mediate different aspects of Notch signalling. In this study, we focus on two zebrafish Notch-dependent hairy/Enhancer-of-split-related transcription factors, Her6 and Her4, which are expressed at the transition zone between presomitic mesoderm and the segmented somites. The results of overexpression/gain-of-function and of morpholino-mediated loss-of-function experiments show that Her6 and Her4 are Notch signalling effectors that feedback on the clock and take part in the maintenance of cyclic gene expression coordination among adjacent cells in the presomitic mesoderm.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping