PUBLICATION
Short interfering RNA-mediated gene targeting in the zebrafish
- Authors
- Dodd, A., Chambers, S.P., and Love, D.R.
- ID
- ZDB-PUB-040312-4
- Date
- 2004
- Source
- FEBS letters 561(1-3): 89-93 (Journal)
- Registered Authors
- Chambers, Steve, Dodd, Andrew, Love, Donald R.
- Keywords
- none
- MeSH Terms
-
- Animals
- Dystrophin/genetics*
- Embryonic and Fetal Development/drug effects
- Gene Silencing/drug effects*
- Histocytochemistry
- Methods
- Models, Animal
- Muscle Fibers, Skeletal/drug effects
- RNA, Small Interfering/chemical synthesis
- RNA, Small Interfering/pharmacology*
- Zebrafish/genetics
- PubMed
- 15013756 Full text @ FEBS Lett.
Citation
Dodd, A., Chambers, S.P., and Love, D.R. (2004) Short interfering RNA-mediated gene targeting in the zebrafish. FEBS letters. 561(1-3):89-93.
Abstract
Short interfering RNAs (siRNAs) have proved to be a useful tool in studying gene function in plants, invertebrates and mammalian systems. Here we report the use of siRNAs for targeting the zebrafish dystrophin gene. This study demonstrates the efficacy of siRNA-based gene silencing in this vertebrate model species, and illustrates the potential of this approach for determining the roles of multiple protein products expressed by a single gene during the early stages of development. In addition this study illustrates the usefulness of zebrafish as a model for muscle disease, and highlights the potential of siRNA-based gene targeting for disease analysis in this model organism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping