PUBLICATION
Immunocytochemistry as a tool for zebrafish developmental neurobiology
- Authors
- Novak, A.E. and Ribera, A.B.
- ID
- ZDB-PUB-031229-12
- Date
- 2003
- Source
- Methods in cell science : an official journal of the Society for In Vitro Biology 25(1-2): 79-83 (Review)
- Registered Authors
- Novak, Alicia, Ribera, Angie
- Keywords
- AEC (3-Amino-9-ethylcarbazole), Double in situ hybridization/immunocytochemistry, Fast Red, Immunocytochemistry, Zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/metabolism
- Fluorescent Dyes/chemistry
- Immunoenzyme Techniques
- Immunohistochemistry/methods*
- In Situ Hybridization, Fluorescence
- Neurons/metabolism*
- Spinal Cord/metabolism*
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 14739591 Full text @ Methods Cell Sci.
Citation
Novak, A.E. and Ribera, A.B. (2003) Immunocytochemistry as a tool for zebrafish developmental neurobiology. Methods in cell science : an official journal of the Society for In Vitro Biology. 25(1-2):79-83.
Abstract
Two methods are presented here that allow clear visualization of antibody localization in zebrafish whole mount preparations, both for immunocytochemistry (ICC) alone and in combination with in situ hybridization (ISH). The first protocol describes ICC performed using a modified permeabilization technique and the chromogen AEC (3-Amino-9-ethylcarbazole). The second protocol describes the co-localization of transcriptional and translational products using a combined ISH/ICC protocol. A fluorescing chromogen (Fast Red, FR) is used to detect mRNA transcripts by ISH, and is combined with ICC that uses a secondary antibody conjugated to a different fluorescent molecule (Alexa 488). These procedures allow the identification of gene expression patterns in cell types identifiable with known antibodies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping