PUBLICATION
The expression of gelatinase A (MMP-2) is required for normal development of zebrafish embryos
- Authors
- Zhang, J., Bai, S., Zhang, X., Nagase, H., and Sarras Jr., M.P.
- ID
- ZDB-PUB-030805-1
- Date
- 2003
- Source
- Development genes and evolution 213(9): 456-463 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Embryo, Nonmammalian/enzymology
- Matrix Metalloproteinase 2/biosynthesis
- Matrix Metalloproteinase 2/genetics*
- Molecular Sequence Data
- RNA, Messenger/metabolism
- Zebrafish/embryology*
- PubMed
- 12898250 Full text @ Dev. Genes Evol.
Citation
Zhang, J., Bai, S., Zhang, X., Nagase, H., and Sarras Jr., M.P. (2003) The expression of gelatinase A (MMP-2) is required for normal development of zebrafish embryos. Development genes and evolution. 213(9):456-463.
Abstract
Gelatinase A, also called matrix metalloproteinase 2 (MMP-2), belongs to the matrix metalloproteinase (MMP) family. MMP-2 cleaves type IV collagen, denatured collagen (gelatin), and other extracellular matrix (ECM) components. MMP-2 has been reported to be involved in a number of biological and pathological processes, but previous studies have not indicated that its expression is essential for early embryogenesis. In the current study, we have utilized zebrafish as a developmental model to study the role of MMP-2 during embryogenesis. We have successfully isolated a zebrafish MMP-2 (zMMP-2) homologue showing over 80% identity and over 90% similarity to its human counterpart. In situ analysis showed that zMMP-2 was expressed as early as the one-cell stage implying a maternal origin during oogenesis, and embryos continued to express zMMP-2 through at least the 72-h stage of development. RT-PCR analysis confirmed the in situ expression pattern and gelatin zymography indicated that a metalloproteinase with the same gel mobility as vertebrate MMP-2 was present in zebrafish embryos. Injection of zMMP-2 antisense morpholino oligonucleotides into 1- to 4-cell embryos resulted in a truncated axis, monitored through 72 h of development indicating that this metalloproteinase plays an important role in zebrafish embryogenesis. Monpholino-induced alterations in development began to be observed at 12 h of embryogenesis based on morphological and axis marker studies. The results obtained in zebrafish are in contrast to murine knockout studies that indicate that MMP-2 does not have a major role in mouse embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping