PUBLICATION
The zebrafish spi1 promoter drives myeloid-specific expression in stable transgenic fish
- Authors
- Ward, A.C., McPhee, D.O., Condron, M.M., Varma, S., Cody, S.H., Onnebo, S.M., Paw, B.H., Zon, L.I., and Lieschke, G.J.
- ID
- ZDB-PUB-030721-4
- Date
- 2003
- Source
- Blood 102(9): 3238-3240 (Journal)
- Registered Authors
- Lieschke, Graham J., McPhee, Dora, Paw, Barry, Varma, Sony, Ward, Alister C., Zon, Leonard I.
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- DNA, Complementary
- Embryo, Nonmammalian
- Green Fluorescent Proteins
- Luminescent Proteins/genetics
- Myeloid Cells/metabolism*
- Promoter Regions, Genetic/genetics*
- Proto-Oncogene Proteins/genetics*
- Trans-Activators/genetics*
- Transgenes
- Zebrafish/genetics*
- PubMed
- 12869502 Full text @ Blood
Citation
Ward, A.C., McPhee, D.O., Condron, M.M., Varma, S., Cody, S.H., Onnebo, S.M., Paw, B.H., Zon, L.I., and Lieschke, G.J. (2003) The zebrafish spi1 promoter drives myeloid-specific expression in stable transgenic fish. Blood. 102(9):3238-3240.
Abstract
The spi1 (pu.1) gene has recently been identified as a useful marker of early myeloid cells in zebrafish. In order to enhance the versatility of this organism as a model for studying myeloid development, the promoter of this gene has been isolated and characterized. Transient transgenesis revealed that a 5.3 kb promoter fragment immediately upstream of the spi1 coding sequence was sufficient to drive expression of EGFP in injected embryos in a manner that largely recapitulated the native spi1 gene expression pattern. This fragment was successfully used to produce a germline transgenic line of zebrafish with EGFP-expressing myeloid cells. These TG(spi1:EGFP)(pA301) transgenic zebrafish represent a valuable tool for further studies of myeloid development and its perturbation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping