PUBLICATION

phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development

Authors
Lawson, N.D., Mugford, J.W., Diamond, B.A., and Weinstein, B.M.
ID
ZDB-PUB-030717-17
Date
2003
Source
Genes & Development   17(11): 1346-1351 (Journal)
Registered Authors
Diamond, Brianne, Lawson, Nathan, Mugford, Joshua, Weinstein, Brant M.
Keywords
none
MeSH Terms
  • Crosses, Genetic
  • Phospholipase C gamma
  • Recombinant Proteins/metabolism
  • Animals, Genetically Modified
  • DNA Primers
  • RNA, Messenger/genetics
  • Arteries/cytology
  • Arteries/embryology*
  • Molecular Sequence Data
  • Genetic Carrier Screening
  • Intercellular Signaling Peptides and Proteins/genetics*
  • Lymphokines/genetics*
  • Type C Phospholipases/genetics*
  • Type C Phospholipases/metabolism
  • Female
  • Animals
  • Zebrafish/embryology*
  • Male
  • Base Sequence
  • Embryo, Nonmammalian/physiology
  • Vascular Endothelial Growth Factors
  • Polymerase Chain Reaction
  • Endothelial Growth Factors/genetics*
  • Vascular Endothelial Growth Factor A
  • Muscle, Smooth, Vascular/cytology
  • Muscle, Smooth, Vascular/embryology
  • Gene Expression Regulation, Developmental*
PubMed
12782653 Full text @ Genes & Dev.
Abstract
In this study, we utilize transgenic zebrafish with fluorescently labeled blood vessels to identify and characterize a mutant (y10) that displays specific defects in the formation of arteries, but not veins. We find that y10 encodes phospholipase C gamma-1 (plcg1), a known effector of receptor tyrosine kinase signaling. We further show that plcg1y10 mutant embryos fail to respond to exogenous Vegf. Our results indicate that Plcg1 functions specifically downstream of the Vegf receptor during embryonic development to govern formation of the arterial system.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping