PUBLICATION
A 90° rotation of the mitotic spindle changes the orientation of mitoses of zebrafish neuroepithelial cells
- Authors
- Geldmacher-Voss, B., Reugels, A.M., Pauls, S., and Campos-Ortega, J.A.
- ID
- ZDB-PUB-030707-11
- Date
- 2003
- Source
- Development (Cambridge, England) 130(16): 3767-3780 (Journal)
- Registered Authors
- Campos-Ortega, Jose, Pauls, Stefan, Reugels, Alexander
- Keywords
- Zebrafish, Neurulation, Mitoses, Orientation, Spindle rotation, Neuroepithelial polarity, ASIP, Histone2A.F/Z:GFP, tau:GFP
- MeSH Terms
-
- Animals
- Biomarkers
- Carrier Proteins/genetics
- Carrier Proteins/metabolism
- Cell Movement/physiology
- Cell Polarity*
- Central Nervous System/cytology
- Central Nervous System/embryology*
- Central Nervous System/physiology
- Epithelial Cells/cytology
- Epithelial Cells/physiology*
- Isoenzymes
- Microinjections
- Mitosis/physiology*
- Oligonucleotides, Antisense/genetics
- Oligonucleotides, Antisense/metabolism
- Protein Kinase C/metabolism
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Recombinant Fusion Proteins/metabolism
- Spindle Apparatus/physiology*
- Transgenes
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 12835393 Full text @ Development
Citation
Geldmacher-Voss, B., Reugels, A.M., Pauls, S., and Campos-Ortega, J.A. (2003) A 90° rotation of the mitotic spindle changes the orientation of mitoses of zebrafish neuroepithelial cells. Development (Cambridge, England). 130(16):3767-3780.
Abstract
In the neural plate and neural tube in the trunk region of the zebrafish embryo, dividing cells are oriented parallel to the plane of the neuroepithelium, while in neural keel/rod, cells divide perpendicular to it. This change in the orientation of mitosis is brought about by a 90° rotation of the mitotic spindle. As the two halves of the neural primordium in keel/rod stage are in apposition, the perpendicular orientation of mitoses in this stage determines that daughter cells become allocated to both sides of the neural tube. To assess the role played by cell junctions in controlling the orientation of dividing cells, we studied the expression of components of adherens and tight junctions in the neuroepithelial cells. We find that these proteins are distributed irregularly at the neural plate stage and become polarised apically in the cell membrane only during the keel/rod stage. The stereotypic orientation of mitoses is perturbed only weakly upon loss of function of the cell junction components ASIP and aPKC{lambda}, suggesting that mitotic orientation depends in part on the integrity of cell junctions and the polarity of the epithelium as a whole. However, the 90° rotation of the spindle does not require perfectly polarised cell junctions between the neuroepithelial cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping