PUBLICATION
The role of Ppt/Wnt5 in regulating cell shape and movement during zebrafish gastrulation
- Authors
- Kilian, B., Mansukoski, H., Barbosa, F.C., Ulrich, F., Tada, M., and Heisenberg, C.P.
- ID
- ZDB-PUB-030408-5
- Date
- 2003
- Source
- Mechanisms of Development 120(4): 467-476 (Journal)
- Registered Authors
- Heisenberg, Carl-Philipp, Kilian, Beate, Mansukoski, Hannu, Tada, Masazumi, Ulrich, Florian
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Lineage
- Gastrula/metabolism
- Gastrula/physiology*
- Gene Expression Regulation, Developmental*
- Glycoproteins/genetics
- Glycoproteins/physiology
- In Situ Hybridization
- Microscopy, Confocal
- Mutation
- Oligonucleotides, Antisense/pharmacology
- Phenotype
- Proto-Oncogene Proteins/physiology*
- Receptors, Neurotransmitter/metabolism
- Signal Transduction
- Time Factors
- Transcription Factors/genetics
- Transcription Factors/physiology*
- Wnt Proteins
- Zebrafish
- Zebrafish Proteins/physiology*
- PubMed
- 12676324 Full text @ Mech. Dev.
Citation
Kilian, B., Mansukoski, H., Barbosa, F.C., Ulrich, F., Tada, M., and Heisenberg, C.P. (2003) The role of Ppt/Wnt5 in regulating cell shape and movement during zebrafish gastrulation. Mechanisms of Development. 120(4):467-476.
Abstract
Wnt genes play important roles in regulating patterning and morphogenesis during vertebrate gastrulation. In zebrafish, slb/wnt11 is required for convergence and extension movements, but not cell fate specification during gastrulation. To determine if other Wnt genes functionally interact with slb/wnt11, we analysed the role of ppt/wnt5 during zebrafish gastrulation. ppt/wnt5 is maternally provided and zygotically expressed at all stages during gastrulation. The analysis of ppt mutant embryos reveals that Ppt/Wnt5 regulates cell elongation and convergent extension movements in posterior regions of the gastrula, while its function in more anterior regions is largely redundant to that of Slb/Wnt11. Frizzled-2 functions downstream of ppt/wnt5, indicating that it might act as a receptor for Ppt/Wnt5 in this process. The characterisation of the role of Ppt/Wnt5 provides insight into the functional diversity of Wnt genes in regulating vertebrate gastrulation movements.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping