PUBLICATION
Analysis of Wnt8 for neural posteriorizing factor by identifying Frizzled 8c and Frizzled 9 as functional receptors for Wnt8
- Authors
- Momoi, A., Yoda, H., Steinbeisser, H., Fagotto, F., Kondoh, H., Kudo, A., Driever, W., and Furutani-Seiki, M.
- ID
- ZDB-PUB-030408-4
- Date
- 2003
- Source
- Mechanisms of Development 120(4): 477-489 (Journal)
- Registered Authors
- Driever, Wolfgang, Furutani-Seiki, Makoto, Kondoh, Hisato
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Blotting, Northern
- Cell Nucleus/metabolism
- Cytoskeletal Proteins/metabolism
- DNA, Complementary/metabolism
- Genes, Dominant
- In Situ Hybridization
- Mesoderm/metabolism
- Molecular Sequence Data
- Mutation
- Neurons/metabolism*
- Phenotype
- Protein Binding
- Protein Structure, Tertiary
- Proteins/genetics
- Proteins/physiology*
- RNA, Messenger/metabolism
- Receptors, Cell Surface/genetics
- Receptors, Cell Surface/physiology*
- Receptors, Neurotransmitter/genetics
- Receptors, Neurotransmitter/physiology*
- Sequence Homology, Amino Acid
- Signal Transduction
- Time Factors
- Trans-Activators/metabolism
- Wnt Proteins
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- beta Catenin
- PubMed
- 12676325 Full text @ Mech. Dev.
Citation
Momoi, A., Yoda, H., Steinbeisser, H., Fagotto, F., Kondoh, H., Kudo, A., Driever, W., and Furutani-Seiki, M. (2003) Analysis of Wnt8 for neural posteriorizing factor by identifying Frizzled 8c and Frizzled 9 as functional receptors for Wnt8. Mechanisms of Development. 120(4):477-489.
Abstract
The dorsal ectoderm of vertebrate gastrula is first specified into anterior fate by an activation signal and posteriorized by a graded transforming signal, leading to the formation of forebrain, midbrain, hindbrain and spinal cord along the anteroposterior (A-P) axis. Transplanted non-axial mesoderm rather than axial mesoderm has an ability to transform prospective anterior neural tissue into more posterior fates in zebrafish. Wnt8 is a secreted factor that is expressed in non-axial mesoderm. To investigate whether Wnt8 is the neural posteriorizing factor that acts upon neuroectoderm, we first assigned Frizzled 8c and Frizzled 9 to be functional receptors for Wnt8. We then, transplanted non-axial mesoderm into the embryos in which Wnt8 signaling is cell-autonomously blocked by the dominant-negative form of Wnt8 receptors. Non-axial mesodermal transplants in embryos in which Wnt8 signaling is cell-autonomously blocked induced the posterior neural markers as efficiently as in wild-type embryos, suggesting that Wnt8 signaling is not required in neuroectoderm for posteriorization by non-axial mesoderm. Furthermore, Wnt8 signaling, detected by nuclear localization of beta-catenin, was not activated in the posterior neuroectoderm but confined in marginal non-axial mesoderm. Finally, ubiquitous over-expression of Wnt8 does not expand neural ectoderm of posterior character in the absence of mesoderm or Nodal-dependent co-factors. We thus conclude that other factors from non-axial mesoderm may be required for patterning neuroectoderm along the A-P axis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping