PUBLICATION
Gelsolin is a dorsalizing factor in zebrafish
- Authors
- Kanungo, J., Kozmik, Z., Swamynathan, S.K., and Piatigorsky, J.
- ID
- ZDB-PUB-030319-11
- Date
- 2003
- Source
- Proceedings of the National Academy of Sciences of the United States of America 100(6): 3287-3292 (Journal)
- Registered Authors
- Piatigorsky, Joram
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- Body Patterning/genetics
- Body Patterning/physiology
- Bone Morphogenetic Proteins/metabolism
- Cornea/embryology
- Cornea/metabolism
- Gelsolin/antagonists & inhibitors
- Gelsolin/genetics
- Gelsolin/physiology*
- Gene Expression Regulation, Developmental
- Glycoproteins/genetics
- Homeodomain Proteins/genetics
- In Situ Hybridization
- Intercellular Signaling Peptides and Proteins*
- Oligodeoxyribonucleotides, Antisense/genetics
- Oligodeoxyribonucleotides, Antisense/pharmacology
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Repressor Proteins/genetics
- Signal Transduction
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/physiology*
- Zebrafish Proteins/genetics
- PubMed
- 12629212 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Kanungo, J., Kozmik, Z., Swamynathan, S.K., and Piatigorsky, J. (2003) Gelsolin is a dorsalizing factor in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 100(6):3287-3292.
Abstract
The gene for gelsolin (an actin-binding, cytoskeletal regulatory protein) was shown earlier to be specialized for high corneal expression in adult zebrafish. We show here that zebrafish gelsolin is required for proper dorsalization during embryogenesis. Inhibition of gelsolin expression by injecting fertilized eggs with a specific morpholino oligonucleotide resulted in a range of concentration-dependent ventralized phenotypes, including those lacking a brain and eyes. These were rescued by coinjection of zebrafish gelsolin or chordin (a known dorsalizing agent) mRNAs, or human gelsolin protein. Moreover, injection of gelsolin mRNA or human gelsolin protein by itself dorsalized the developing embryos, often resulting in axis duplication. Injection of the gelsolin-specific morpholino oligonucleotide enhanced the expression of Vent mRNA, a ventral marker downstream of bone morphogenetic proteins, whereas injection of gelsolin mRNA enhanced the expression of chordin and goosecoid mRNAs, both dorsal markers. Our results indicate that gelsolin also modulates embryonic dorsal/ventral pattern formation in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping