PUBLICATION
Two tcf3 genes cooperate to pattern the zebrafish brain
- Authors
- Dorsky, R.I., Itoh, M., Moon, R.T., and Chitnis, A.
- ID
- ZDB-PUB-030319-1
- Date
- 2003
- Source
- Development (Cambridge, England) 130(9): 1937-1947 (Journal)
- Registered Authors
- Chitnis, Ajay, Dorsky, Richard, Itoh, Motoyuki, Moon, Randall T.
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Brain/embryology*
- Cytoskeletal Proteins
- HMGB Proteins/genetics*
- HMGB Proteins/metabolism
- Molecular Sequence Data
- Mutation
- Phenotype
- Proteins/metabolism
- RNA, Antisense
- TCF Transcription Factors
- Transcription Factor 7-Like 1 Protein
- Transcription Factors/genetics*
- Transcription Factors/metabolism
- Wnt Proteins
- Zebrafish/embryology*
- Zebrafish Proteins
- PubMed
- 12642497 Full text @ Development
Citation
Dorsky, R.I., Itoh, M., Moon, R.T., and Chitnis, A. (2003) Two tcf3 genes cooperate to pattern the zebrafish brain. Development (Cambridge, England). 130(9):1937-1947.
Abstract
Caudalizing factors operate in the context of Wnt/beta-catenin signaling to induce gene expression in discrete compartments along the rostral-caudal axis of the developing vertebrate nervous system. In zebrafish, basal repression of caudal genes is achieved through the function of Headless (Hdl), a Tcf3 homolog. In this study, we show that a second Tcf3 homolog, Tcf3b, limits caudalization caused by loss of Hdl function and although this Lef/Tcf family member can rescue hdl mutants, Lef1 cannot. Wnts can antagonize repression mediated by Tcf3 and this derepression is dependent on a Tcf3 beta-catenin binding domain. Systematic changes in gene expression caused by reduced Tcf3 function help predict the shape of a caudalizing activity gradient that defines compartments along the rostral-caudal axis. In addition, Tcf3b has a second and unique role in the morphogenesis of rhombomere boundaries, indicating that it controls multiple aspects of brain development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping