PUBLICATION
Zinc finger protein too few controls the development of monoaminergic neurons
- Authors
- Levkowitz, G., Zeller, J., Sirotkin, H.I., French, D., Schilbach, S., Hashimoto, H., Hibi, M., Talbot, W.S., and Rosenthal, A.
- ID
- ZDB-PUB-030116-6
- Date
- 2003
- Source
- Nature Neuroscience 6(1): 28-33 (Journal)
- Registered Authors
- Hashimoto, Hisashi, Hibi, Masahiko, Levkowitz, Gil, Sirotkin, Howard, Talbot, William S., Zeller, Joerg
- Keywords
- none
- MeSH Terms
-
- Dopamine/metabolism
- Male
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental/genetics
- Brain/cytology
- Brain/embryology*
- Brain/metabolism
- Apoptosis/genetics
- Zebrafish
- Peptides*
- Signal Transduction/genetics
- Neurons/cytology
- Neurons/metabolism*
- Zinc Fingers/genetics
- Cell Differentiation/genetics*
- Biogenic Monoamines/metabolism*
- Brain Tissue Transplantation
- Anti-Bacterial Agents/chemical synthesis*
- Carrier Proteins/genetics
- Carrier Proteins/isolation & purification*
- Female
- Chromosome Mapping
- Repressor Proteins/genetics
- Repressor Proteins/isolation & purification
- Zebrafish Proteins*
- Animals
- Serotonin/metabolism
- Stem Cells/cytology
- Stem Cells/metabolism*
- PubMed
- 12469125 Full text @ Nat. Neurosci.
Citation
Levkowitz, G., Zeller, J., Sirotkin, H.I., French, D., Schilbach, S., Hashimoto, H., Hibi, M., Talbot, W.S., and Rosenthal, A. (2003) Zinc finger protein too few controls the development of monoaminergic neurons. Nature Neuroscience. 6(1):28-33.
Abstract
The mechanism controlling the development of dopaminergic (DA) and serotonergic (5HT) neurons in vertebrates is not well understood. Here we characterized a zebrafish mutant-too few (tof)-that develops hindbrain 5HT and noradrenergic neurons, but does not develop hypothalamic DA and 5HT neurons. tof encodes a forebrain-specific zinc finger transcription repressor that is homologous to the mammalian Fezl (forebrain embryonic zinc finger-like protein). Mosaic and co-staining analyses showed that fezl was not expressed in DA or 5HT neurons and instead controlled development of these neurons non-cell-autonomously. Both the eh1-related repressor motif and the second zinc finger domain were necessary for tof function. Our results indicate that tof/fezl is a key component in regulating the development of monoaminergic neurons in the vertebrate brain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping