PUBLICATION
Neurogenin1 defines zebrafish cranial sensory Ganglia precursors
- Authors
- Andermann, P., Ungos, J., and Raible, D.W.
- ID
- ZDB-PUB-021106-12
- Date
- 2002
- Source
- Developmental Biology 251(1): 45-58 (Journal)
- Registered Authors
- Andermann, Peter, Raible, David, Ungos, Josette
- Keywords
- trigeminal placode; lateral line; epibranchial placode; otic placode; neurogenin; bHLH transcription factor; morpholino oligonucleotides
- MeSH Terms
-
- Animals
- Basic Helix-Loop-Helix Transcription Factors
- Cell Differentiation/genetics*
- Cell Movement/genetics
- Ganglia/embryology*
- Ganglia/physiology
- Gene Expression Regulation, Developmental
- Helix-Loop-Helix Motifs/genetics
- Nerve Tissue Proteins/genetics*
- Transcription Factors/genetics*
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish Proteins*
- PubMed
- 12413897 Full text @ Dev. Biol.
Citation
Andermann, P., Ungos, J., and Raible, D.W. (2002) Neurogenin1 defines zebrafish cranial sensory Ganglia precursors. Developmental Biology. 251(1):45-58.
Abstract
Cells delaminate from epithelial placodes to form sensory ganglia in the vertebrate head. We describe the formation of cranial neurogenic placodes in the zebrafish, Danio rerio, using bHLH transcription factors as molecular markers. A single neurogenin gene, neurogenin1 (ngn1), is required for the development of all zebrafish cranial ganglia, which contrasts with other described vertebrates. Expression of ngn1 delineates zebrafish ganglionic placodes, including trigeminal, lateral line, and epibranchial placodes. In addition, ngn1 is expressed in a subset of cells within the otic vesicle that will delaminate to form the octaval (statoacoustic) ganglion. The trigeminal placode is the first to differentiate, and forms just lateral and adjacent to the neural crest. Expression of ngn1 is transient and prefigures expression of a related bHLH transcription factor, neuroD. Interfering with ngn1 function using a specific antisense morpholino oligonucleotide blocks differentiation of all cranial ganglia but not associated glial cells. Lateral line sensory neuromasts develop independently of ngn1 function, suggesting that two derivatives of lateral line placodes, ganglia and migrating primordia, are under separate genetic control.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping