PUBLICATION
Specification of an anterior neuroectoderm patterning by Frizzled8a-mediated Wnt8b signalling during late gastrulation in zebrafish
- Authors
- Kim, S.-H., Shin, J., Park, H.-C., Yeo, S.-Y., Hong, S.-K., Han, S., Rhee, M., Kim, C.-H., Chitnis, A.B., and Huh, T.-L.
- ID
- ZDB-PUB-020913-5
- Date
- 2002
- Source
- Development (Cambridge, England) 129(19): 4443-4455 (Journal)
- Registered Authors
- Chitnis, Ajay, Hong, Sung-Kook, Huh, Tae-Lin, Kim, Cheol-Hee, Kim, Seok-Hyung, Park, Hae-Chul, Shin, Jimann, Yeo, Sang-Yeob
- Keywords
- Fz8a, Wnt8b, Anterior neuroectoderm, Brain patterning, Eye primordia, Diencephalon, Midbrain, Zebrafish
- MeSH Terms
-
- Animals
- Body Patterning/physiology*
- Brain/embryology*
- Ectoderm
- Eye/embryology
- Gastrula/physiology
- Gene Expression
- Gene Targeting
- Genes, Overlapping
- Proteins/genetics
- Proteins/metabolism*
- Proto-Oncogene Proteins/metabolism
- Receptors, Cell Surface/genetics
- Receptors, Cell Surface/metabolism*
- Signal Transduction*
- Wnt Proteins
- Xenopus Proteins*
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish Proteins*
- PubMed
- 12223403 Full text @ Development
Citation
Kim, S.-H., Shin, J., Park, H.-C., Yeo, S.-Y., Hong, S.-K., Han, S., Rhee, M., Kim, C.-H., Chitnis, A.B., and Huh, T.-L. (2002) Specification of an anterior neuroectoderm patterning by Frizzled8a-mediated Wnt8b signalling during late gastrulation in zebrafish. Development (Cambridge, England). 129(19):4443-4455.
Abstract
Wnts have been shown to provide a posteriorizing signal that has to be repressed in the anterior neuroectoderm for normal anteroposterior (AP) patterning. We have previously identified a zebrafish frizzled8a (fz8a) gene expressed in the presumptive anterior neuroectoderm as well as prechordal plate at the late gastrula stage. We have investigated the role of Fz8a-mediated Wnt8b signalling in anterior brain patterning in zebrafish. We show that in zebrafish embryos: (1) Wnt signalling has at least two different stage-specific posteriorizing activities in the anterior neuroectoderm, one before mid-gastrulation and the other at late gastrulation; (2) Fz8a plays an important role in mediating anterior brain patterning; (3) Wnt8b and Fz8a can functionally interact to transmit posteriorizing signals that determine the fate of the posterior diencephalon and midbrain in late gastrula embryos; and (4) Wnt8b can suppress fz8a expression in the anterior neuroectoderm and potentially affect the level and/or range of Wnt signalling. In conclusion, we suggest that a gradient of Fz8a-mediated Wnt8b signalling may play crucial role in patterning the posterior diencephalon and midbrain regions in the late gastrula.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping