PUBLICATION
Structure, developmental expression, and physiological regulation of zebrafish IGF binding protein-1
- Authors
- Maures, T.J. and Duan, C.
- ID
- ZDB-PUB-020701-9
- Date
- 2002
- Source
- Endocrinology 143(7): 2722-2731 (Journal)
- Registered Authors
- Duan, Cunming
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Cloning, Molecular
- Databases, Factual
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental/physiology*
- Hypoxia/metabolism
- In Situ Hybridization
- Insulin-Like Growth Factor Binding Protein 1/biosynthesis*
- Insulin-Like Growth Factor Binding Protein 1/genetics*
- Insulin-Like Growth Factor Binding Protein 1/physiology
- Liver/embryology
- Liver/metabolism
- Molecular Sequence Data
- Nutritional Physiological Phenomena
- RNA, Messenger/biosynthesis
- RNA, Messenger/genetics
- Reverse Transcriptase Polymerase Chain Reaction
- Tissue Distribution
- Zebrafish
- PubMed
- 12072407 Full text @ Endocrinology
Citation
Maures, T.J. and Duan, C. (2002) Structure, developmental expression, and physiological regulation of zebrafish IGF binding protein-1. Endocrinology. 143(7):2722-2731.
Abstract
The biological activity and availability of IGFs are regulated by a group of secreted proteins that belong to the IGF-binding protein (IGFBP) gene family. Although six IGFBPs have been identified and studied in mammals, their nonmammalian orthologs remain poorly defined. In this study, we cloned and characterized the full-length zebrafish IGFBP-1. Sequence analysis indicated that its structure is homologous to mammalian IGFBP-1. Using in situ RNA hybridization and RT-PCR, we discovered that IGFBP-1 mRNA was present in all early embryonic stages albeit at very low levels. IGFBP-1 mRNA was initially expressed in multiple embryonic tissues but became restricted to the liver shortly after hatching. In the adult stage, IGFBP-1 mRNA was found only in the liver at low levels. Prolonged food deprivation caused a significant increase in the hepatic IGFBP-1 mRNA levels, and refeeding restored the IGFBP-1 mRNA to the basal levels. When adult fish or embryos were subjected to hypoxic conditions, the IGFBP-1 mRNA expression increased dramatically. Intriguingly, the hypoxia-induced IGFBP-1 expression operated in different embryonic tissues in a developmental-stage-dependent manner. In early embryos, hypoxia-stimulated IGFBP-1 mRNA expression in the pharyngeal arches, ventricle, atrium, and brain. After hatching, the hypoxia-induced IGFBP-1 expression became liver specific. These results not only provide new information about the structural conservation, developmental expression, and physiological regulation of the IGFBP-1 gene but also present the opportunity to elucidate the developmental role of IGFBP-1 using a unique vertebrate model organism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping