PUBLICATION

Tbx24, encoding a T-box protein, is mutated in the zebrafish somite-segmentation mutant fused somites

Authors
Nikaido, M., Kawakami, A., Sawada, A., Furutani-Seiki, M., Takeda, H., and Araki, K.
ID
ZDB-PUB-020605-4
Date
2002
Source
Nature Genetics   31(2): 195-199 (Journal)
Registered Authors
Araki, Kazuo, Furutani-Seiki, Makoto, Kawakami, Atsushi, Nikaido, Masataka, Sawada, Atsushi, Takeda, Hiroyuki
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Cell Differentiation/genetics
  • Chromosome Mapping
  • Gene Expression Regulation, Developmental
  • Molecular Sequence Data
  • Mutation*
  • Somites/cytology
  • Somites/physiology*
  • T-Box Domain Proteins/genetics*
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
PubMed
12021786 Full text @ Nat. Genet.
Abstract
Somites are fundamental structures within the paraxial mesoderm of the vertebrate embryo that give rise to the vertebrae and muscle of the trunk and tail. Studies of knockout mice and gene expression analyses have shown that the Notch pathway is crucial in establishing the reiterative pattern of somites. A large-scale screen in zebrafish previously identified five mutants that show abnormalities in somite boundary formation. Four have essentially the same phenotype, with posterior somite defects and neuronal hyperplasia; recent work has suggested that genes affected in these mutants encode components of the Notch signaling cascade. The fifth mutant, fused somites (fss), shows a different phenotype characterized by complete lack of somite formation along the entire antero-posterior axis. Gene expression and phenotypic analyses in mutant embryos have implicated Fss in somite formation independent of Notch signaling, suggesting the presence of a new pathway regulating somite boundary formation. We show here that the fss gene encodes a T-box transcription factor that is expressed in intermediate to anterior presomitic mesoderm (PSM) and is involved in PSM maturation.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping