PUBLICATION
The planar cell-polarity gene stbm regulates cell behaviour and cell fate in vertebrate embryos
- Authors
- Park, M. and Moon, R.T.
- ID
- ZDB-PUB-020219-18
- Date
- 2002
- Source
- Nature cell biology 4(1): 20-25 (Journal)
- Registered Authors
- Moon, Randall T., Park, Maiyon
- Keywords
- none
- MeSH Terms
-
- Adaptor Proteins, Signal Transducing
- Amino Acid Sequence
- Animals
- Cell Movement/genetics*
- Cell Polarity/genetics*
- Cytoskeletal Proteins/physiology
- Drosophila
- Drosophila Proteins*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/physiology
- Gastrula/cytology
- Gastrula/physiology
- Gene Expression Regulation, Developmental/physiology
- Membrane Proteins/genetics*
- Membrane Proteins/physiology
- Mice
- Molecular Sequence Data
- Phosphoproteins/physiology
- Sequence Alignment
- Signal Transduction
- Trans-Activators*
- Xenopus/embryology*
- Xenopus/genetics
- Xenopus Proteins*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins*
- beta Catenin
- PubMed
- 11780127 Full text @ Nat. Cell Biol.
Citation
Park, M. and Moon, R.T. (2002) The planar cell-polarity gene stbm regulates cell behaviour and cell fate in vertebrate embryos. Nature cell biology. 4(1):20-25.
Abstract
The gene strabismus (stbm)/Van Gogh (Vang) functions in the planar cell-polarity pathway in Drosophila. As the existence of such a pathway in vertebrates has not been firmly established, we investigated the functions and signalling activities encoded by stbm in vertebrate embryos. In regard to cell fate, inhibition of Stbm function in zebrafish embryos leads to reduction of anterior neural markers, whereas gain of function leads to a rise in the levels of these markers. In regard to cell behaviour, both gain-of-function and loss-of-function assays reveal a role for Stbm in mediating cell movements during gastrulation. Mechanistically, Stbm inhibits Wnt-mediated activation of betacatenin-dependent transcription while promoting phosphorylation of c-Jun- and AP-1-dependent transcription. This complex effect on intracellular signalling pathways probably involves dishevelled (dsh), as Stbm was found to interact with the Dsh protein, and as Dsh is known to function in both planar cell-polarity and beta-catenin pathways in Drosophila.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping