PUBLICATION

A zebrafish nanos related gene is essential for the development of primordial germ cells

Authors
Köprunner, M., Thisse, C., Thisse, B., and Raz, E.
ID
ZDB-PUB-011109-1
Date
2001
Source
Genes & Development   15(21): 2877-2885 (Journal)
Registered Authors
Köprunner, Marion, Raz, Erez, Thisse, Bernard, Thisse, Christine
Keywords
nanos; germ line; PGC; germ plasm; maternal RNA; cell migration; zebrafish
MeSH Terms
  • Time Factors
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • RNA-Binding Proteins*
  • Nitric Oxide Synthase Type I
  • Sequence Homology, Amino Acid
  • Animals
  • Drosophila Proteins*
  • In Situ Hybridization
  • RNA/metabolism
  • Zebrafish
  • Gene Expression Regulation, Developmental
  • Plasmids/metabolism
  • Amino Acid Sequence
  • Molecular Sequence Data
  • Cell Survival
  • Cell Movement
  • Germ Cells/metabolism*
  • Nitric Oxide Synthase/genetics*
  • Nitric Oxide Synthase/physiology*
  • Insect Proteins/genetics*
  • Insect Proteins/physiology*
PubMed
11691838 Full text @ Genes & Dev.
Abstract
Asymmetrically distributed cytoplasmic determinants collectively termed germ plasm have been shown to play an essential role in the development of primordial germ cells (PGCs). Here, we report the identification of a nanos-like (nanos1) gene, which is expressed in the germ plasm and in the PGCs of the zebrafish. We find that several mechanisms act in concert to restrict the activity of Nanos1 to the germ cells including RNA localization and control over the stability and translatability of the RNA. Reducing the level of Nanos1 in zebrafish embryos revealed an essential role for the protein in ensuring proper migration and survival of PGCs in this vertebrate model organism.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping