PUBLICATION

The zygotic expression of zebrafish trebf during embryogenesis is restricted to the embryonic shield and its derivatives

Authors
Liang, L.Y., Li, M., Wang, Y., Zhao, C.T., Zhao, Z.X., and Meng, A.M.
ID
ZDB-PUB-011108-5
Date
2001
Source
Development genes and evolution   211(8-9): 445-448 (Journal)
Registered Authors
Meng, Anming, Wang, Yihong
Keywords
zebrafish; Trebf; hatching gland; notochord
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Conserved Sequence
  • DNA-Binding Proteins/chemistry
  • Embryonic Structures/metabolism*
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Humans
  • Mice
  • Molecular Sequence Data
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Sequence Homology, Amino Acid
  • Transcription Factors/chemistry
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics*
PubMed
11685580 Full text @ Dev. Genes Evol.
Abstract
We have identified a zebrafish gene trebf that shows an overall similarity of 51.5% and 55.1% in amino acid sequence with human and mouse TREB5/hXBP-1/HTF, respectively. Northern hybridization reveals that trebf mRNA is expressed throughout the life cycle of zebrafish. Whole-mount in situ hybridization detects an expression pattern of trebf in zebrafish that is very different from that of mammalian TREB5. Just before the onset of gastrulation, trebf transcripts are accumulated in the dorsal blastoderm margin and subsequently in the forming organizer. During gastrulation, the trebf-positive cells in the organizer migrate toward the animal pole and form two distinct domains. The cells in the anterior leading expression domain ultimately differentiate into hatching gland, whereas those in the following domain form the notochord.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping