PUBLICATION
The zebrafish neckless mutation reveals a requirement for raldh2 in mesodermal signals that pattern the hindbrain
- Authors
- Begemann, G., Schilling, T.F., Rauch, G.J., Geisler, R., and Ingham, P.W.
- ID
- ZDB-PUB-011002-4
- Date
- 2001
- Source
- Development (Cambridge, England) 128(16): 3081-3094 (Journal)
- Registered Authors
- Begemann, Gerrit, Geisler, Robert, Ingham, Philip, Rauch, Gerd-Jörg, Schilling, Tom
- Keywords
- zebrafish; anteroposterior patterning; vitamin A deficiency; retinoic acid; retinoic acid receptor; craniofacial development; neural crest; raldh2; hoxb4
- MeSH Terms
-
- Zebrafish Proteins/agonists
- Models, Genetic
- Tretinoin/metabolism
- Tretinoin/pharmacology
- RNA, Messenger/metabolism
- Cloning, Molecular
- Neural Crest/embryology
- Mesoderm/metabolism*
- Models, Biological
- Aldehyde Oxidoreductases/genetics*
- Aldehyde Oxidoreductases/metabolism
- Animals
- In Situ Hybridization
- Genetic Linkage
- Molecular Sequence Data
- Sequence Homology, Amino Acid
- Signal Transduction
- Retinal Dehydrogenase
- Transcription Factors/metabolism
- Receptors, Retinoic Acid/metabolism
- In Situ Nick-End Labeling
- Mutation*
- Ectoderm/metabolism
- Phenotype
- Amino Acid Sequence
- Rhombencephalon/embryology
- Rhombencephalon/metabolism*
- Zebrafish
- Notochord/embryology
- Homeodomain Proteins/metabolism
- PubMed
- 11688558 Full text @ Development
Citation
Begemann, G., Schilling, T.F., Rauch, G.J., Geisler, R., and Ingham, P.W. (2001) The zebrafish neckless mutation reveals a requirement for raldh2 in mesodermal signals that pattern the hindbrain. Development (Cambridge, England). 128(16):3081-3094.
Abstract
We describe a new zebrafish mutation, neckless, and present evidence that it inactivates retinaldehyde dehydrogenase type 2, an enzyme involved in retinoic acid biosynthesis. neckless embryos are characterised by a truncation of the anteroposterior axis anterior to the somites, defects in midline mesendodermal tissues and absence of pectoral fins. At a similar anteroposterior level within the nervous system, expression of the retinoic acid receptor a and hoxb4 genes is delayed and significantly reduced. Consistent with a primary defect in retinoic acid signalling, some of these defects in neckless mutants can be rescued by application of exogenous retinoic acid. We use mosaic analysis to show that the reduction in hoxb4 expression in the nervous system is a non-cell autonomous effect, reflecting a requirement for retinoic acid signalling from adjacent paraxial mesoderm. Together, our results demonstrate a conserved role for retinaldehyde dehydrogenase type 2 in patterning the posterior cranial mesoderm of the vertebrate embryo and provide definitive evidence for an involvement of endogenous retinoic acid in signalling between the paraxial mesoderm and neural tube.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping