PUBLICATION
Isolation and characterization of posteriorly restricted genes in the zebrafish gastrula
- Authors
- Sagerström, C.G., Kao, B.A., Lane, M.E., and Sive, H.
- ID
- ZDB-PUB-010423-6
- Date
- 2001
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 220(4): 402-408 (Journal)
- Registered Authors
- Lane, Mary Ellen, Sagerström, Charles, Sive, Hazel
- Keywords
- subtractive hybridization; cloning; cDNA; patterning; ectoderm; hindbrain; zebrafish; zinc finger; homeobox; transcription factor; gene; Danio rerio; neural; axis
- MeSH Terms
-
- Animals
- Cloning, Molecular
- DNA, Complementary/metabolism
- Genetic Techniques*
- Nucleic Acid Hybridization
- Sequence Analysis, DNA
- Time Factors
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zinc Fingers
- PubMed
- 11307172 Full text @ Dev. Dyn.
Citation
Sagerström, C.G., Kao, B.A., Lane, M.E., and Sive, H. (2001) Isolation and characterization of posteriorly restricted genes in the zebrafish gastrula. Developmental Dynamics : an official publication of the American Association of Anatomists. 220(4):402-408.
Abstract
In order to understand anteroposterior axis formation in vertebrates, we have used subtractive hybridization to clone genes expressed posteriorly in the zebrafish gastrula-stage embryo. Here we report the initial characterization of eight clones isolated from this screen. We find that all eight genes are expressed in posteriorly restricted domains, suggesting that they are involved in regulating posterior development during zebrafish embryogenesis
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping