PUBLICATION
cDNA sequence and tissue-specific expression of a basic liver-type fatty acid binding protein in adult zebrafish (Danio rerio)
- Authors
- Denovan-Wright, E.M., Pierce, M., Sharma, M.K., and Wright, J.M.
- ID
- ZDB-PUB-000622-5
- Date
- 2000
- Source
- BBA Gene Structure and Expression 1492(1): 227-232 (Journal)
- Registered Authors
- Sharma, Mukesh, Wright, Jonathan M.
- Keywords
- liver basic fatty acid binding protein; zebrafish; cDNA cloning; in situ hybridization; Northern analysis
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Carrier Proteins/biosynthesis
- Carrier Proteins/genetics*
- Cloning, Molecular
- DNA, Complementary/analysis
- Fatty Acid-Binding Proteins
- Gene Expression
- Humans
- In Situ Hybridization
- Liver/metabolism*
- Molecular Sequence Data
- Neoplasm Proteins*
- Nerve Tissue Proteins*
- Polymerase Chain Reaction
- Sequence Homology, Amino Acid
- Tumor Suppressor Proteins*
- Zebrafish
- Zebrafish Proteins*
- PubMed
- 11004494 Full text @ BBA Gene Structure and Expression
Citation
Denovan-Wright, E.M., Pierce, M., Sharma, M.K., and Wright, J.M. (2000) cDNA sequence and tissue-specific expression of a basic liver-type fatty acid binding protein in adult zebrafish (Danio rerio). BBA Gene Structure and Expression. 1492(1):227-232.
Abstract
We have determined the nucleotide sequence for a cDNA clone derived from zebrafish (Danio rerio) that codes for a fatty acid binding protein (FABP). Amino acid sequence similarity of the zebrafish FABP was highest to FABPs isolated from the livers of catfish, chicken, salamander and iguana. The open-reading frame of the zebrafish FABP cDNA codes for a protein of 14.0 kDa with a calculated isoelectric point of 8.8. The zebrafish liver-type FABP (L-FABP) cDNA hybridized to single restriction fragments of total zebrafish genomic DNA digested with the restriction endonucleases HaeIII or EcoRI suggesting that a single copy of the L-FABP gene is present in the zebrafish genome. Northern blot analysis demonstrated that the zebrafish L-FABP cDNA hybridized to a transcript of 700 nucleotides in total zebrafish RNA. In situ hybridization and emulsion autoradiography revealed that the L-FABP was expressed exclusively in the liver of the adult zebrafish. Based on amino acid sequence similarity, the isoelectric point and its tissue-specific pattern of expression, we conclude that this zebrafish FABP belongs to the basic liver-type FABPs only found, thus far, in non-mammalian vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping