Biography and Research Interest

Publications

Non-Zebrafish Publications

36. Mutagenesis of E477 or K505 in the B' domain of human topoisomerase IIb increases the requirement for magnesium ions during strand passage. Katherine L.West, Emma L.Meczes. Ronald Thorn, Richard Marshall and C. A. Austin (1999) Submitted.
35. Murine transgenic cells lacking DNA topoisomerase IIb are resistant to acridines and mitoxantrone: analysis of cyctotoxicity and cleavable complex formation. Fiona Errington, Elaine Willmore, Mike J. Tilby, L.Li, Gloria Li, Wei Li, Bruce C. Baguley and Caroline A Austin. (1999) Submitted
34. Camptothecin stabilised topoisomerase I-DNA complexes in leukaemia cells visualised and quantified in situ by the TARDIS assay. Kay Padget, Ross Carr, Andrew D.J.Pearson, Mike J.Tilby and Caroline A Austin. (1999) In press.
33. Molecular cloning and characterization of the human topoisomerase IIalpha and IIbeta genes: evidence for isoform evolution through gene duplication. J.H.Sng, V.Heaton, M.Bell, P.Mani, C.A.Austin and L.M.Fisher. (1999) Biochim Biophys Acta 1444: 395-406.
32. Carbamate analogues of amsacrine active against non-cycling cycles:relative activity against topoisomerases IIa and IIb. Rozalind Turnbull, Emma Meczes, Bruce Baguley, M.Perenna Rogers, Richard. B.Lock, Graeme J. Finlay and C. A. Austin. (1999) Cancer Chemotherapy and Pharmacology in press
31. Human DNA topoisomerase IIb binds and cleaves four-way junction DNA in vitro. K.L.West and C.A.Austin (1999) NAR 27: 984-992.
30. Nuclear distribution of human DNA topoisomerase IIb: A nuclear targeting signal resides in the 116-residue C-terminal tail. I.G.Cowell, E.Willmore, D.Charlton, K.Marsh, E.Jazwari, L.Mark Fisher and C.A.Austin. (1998) Experimental Cell Research 243:232-240.
29. Etoposide targets topoisomerase IIa and IIb in leukaemic cells: isoform specific cleavable complexes visualised and quantified in situ by a novel immunofluorescence technique. E.Willmore, A.J. Frank, K.Padget, M.J.Tilby and C.A.Austin. (1998) Molecular Pharmacology 53:78-85.
28. Human DNA topoisomerase IIa dependent DNA cleavage and yeast cell killing by anthracyclines analogs. M.Binaschi, R,Faninosi, C.A.Austin, L.M.Fisher, F.Zunino, and G.Capranico.(1998) Cancer Res 58:1886-1892.
27.Eukaryotic Topoisomerase IIb C.A.Austin and K.L.Marsh (1998) Bioessays, 20:215-226.
26. Anticancer drug action on topoisomerase II: role of the ternary DNA complex in drug sensitivity
and resistance L.M.Fisher, G.Capranico and C.A. Austin (1998). in 'Cancer research supported under BIOMED 1', ed S S Baig, pp164-170, IOS Press, Amsterdam.
25. Site directed mutagenesis of human topoisomerase IIb. Katherine L.Marsh, Emma L.Meczes. Ronald Thorn, Richard Marshall and C.A. Austin. (1997) Biochemical Society Transactions 25:S638.
24. A survey of final year undergraduate laboratory projects in Biochemistry and related degrees in Great Britain. C.A.Austin (1997) Biochemical Education 25: 12-14.
23. Self-association of chromodomain peptides I.G.Cowell and C.A.Austin (1997) Biochim.Biophys.Acta. 1307: 239-247.
22.Complementation of temperature sensitive topoisomerase II mutations in S.cerevisiae by a human TOP2b construct allows study of topoisomerase IIb inhibitors in yeast. E.L.Meczes, K.L.Marsh, L.M.Fisher, M.P.Rogers, and C.A.Austin (1997) Cancer Chemotherapy and Pharmacology 39: 367-375.
21. Threonine 1342 in Human topoisomerase IIa is phosphorylated throughout the cell cycle. R.Ishida, M.Iwai, K.L.Marsh, C.A.Austin, T.Yano, M.Shibata, N.Nozaki and A.Hara.(1996) J.Biol.Chem 271:30077-30082.
20. Drug sensitivity and sequence specificity of human recombinant DNA topoisomerase IIa and b. M.Cornarotti, S.Tinelli, E.Willmore, F.Zunino, L.M.Fisher, C.A.Austin and G.Capranico. (1996) Molecular Pharmacology 50:1463-1471.
19. Amsarcrine promoted DNA cleavage site determinants for the two human DNA topoisomerase II isoforms, a and b. K.L.Marsh, E.Willmore, S.Tinelli, M.Cornarotti, E.L.Meczes, G.Capranico, L.M.Fisher and C.A.Austin (1996) Biochemical Pharmacology 52: 1675-1685.
18. Analysis of foetal expression sites of human DNA topoisomerase II * and * mRNAs by in situ hybridisation. DW Zandvliet, AM Hanby, C.A.Austin, I Clark, KL Marsh, R Poulsom and NA Wright. (1996) Biochem.Biophys.Acta.1307: 239-247.
17. Position-specific effects of base mismatch on mammalian topoisomerase II DNA cleaving activity. M.Bigioni, F. Zunino, S.Tinelli, C.A.Austin, E.Willmore, G.Capranico. (1996) Biochemistry 35: 153-159.
16. Expression, domain structure and enzymatic properties of an active recombinant human DNA topoisomerase II* C.A.Austin, K.L.Marsh, R.A.Wasserman, E.Willmore, P.J.Sayer , J.C.Wang and L.M.Fisher (1995) J. Biol. Chem. 270: 15739-15746.
15. The use of yeast in the study of anticancer drugs targeting DNA topoisomerases: Expression of a functional recombinant human DNA topoisomerase II* in Yeast. R.A.Wasserman, C.A.Austin, L.M.Fisher and J.C.Wang. (1993) Cancer Research 53:3591-3596.
14. Novel HeLa topoisomerase II is the IIß isoform: complete coding sequence and homology with other type II topoisomerases. C.A.Austin, J.H.Sng, S.Patel and L.M.Fisher. (1993) Biochim. Biophys. Acta 1172:283-291.
13. Different Patterns of Gene Expression of DNA Topoisomerase II Isoenzymes in Differentiated Tissues during Murine Development. G.Capranico, S.Tinelli, C.A.Austin, M.Fisher and F.Zunino (1992) Biochim. Biophys. Acta 1132: 43-48.
12. Baicalein and related cytotoxic flavonoids unwind DNA and are novel inducers of topoisomerase II mediated DNA cleavage. C.A.Austin, S.Patel, K.Ono, H.Nakane and L.M.Fisher. (1992) Biochemical Journal 282: 883-889.
11. DNA Supercoiling and relaxation by the ATP dependent DNA topoisomerases. L.M.Fisher, C.A Austin, R.Hopewell, E.E.C.Margerrison, M.Oram, S.Patel, J.Sng and S.Sreedharan. (1992) Phil.Trans.Royal Society 336:83-91.
10. Isolation and characterisation of a human cDNA clone encoding a novel DNA topoisomerase II homologue from HeLa cells. C.A.Austin and L.M.Fisher (1990) FEBS Letters 266: 115-117.