Person
Hughes, Simon M.
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Biography and Research Interest
OBJECTIVES:
To elucidate the interaction of genetics and environment in regulating phenotype of a specific tissue: striated muscle. To understand the formation, maintenance, growth and repair of fast and slow muscle fibres, their attachments and innervation, in their anatomical context. In a word: to study cell biology in vivo.
ORGANISMS:
We work on zebrafish (mainly), Xenopus, chick and mouse muscle development, growth and repair. We also study effects of environment such as training, exercise or aging.
METHODOLOGY:
Molecular developmental genetics and cell biology. Strong emphasis on development of in vivo imaging.
BIOGRAPHY:
Ph.D., 1980-1984, Cambridge University, Dr. Martin Brand - Visual transduction in retinal rod cells.
Postdoc, 1985-1987, University College London, Professor Martin Raff - Glial cell development in optic nerve.
Postdoc, 1987-1991, Stanford University, Professor Helen Blau - Mammalian muscle development.
Principle Investigator, 1992-2002, MRC Muscle and Cell Motility Unit and Developmental Biology Research Centre, King's College London.
Principle Investigator, 2002-date, MRC External Scientific Staff; Professor of Developmental Cell Biology, Randall Division for Cell and Molecular Biophysics, King's College London and member of MRC Centre for Developmental Neurobiology.
To elucidate the interaction of genetics and environment in regulating phenotype of a specific tissue: striated muscle. To understand the formation, maintenance, growth and repair of fast and slow muscle fibres, their attachments and innervation, in their anatomical context. In a word: to study cell biology in vivo.
ORGANISMS:
We work on zebrafish (mainly), Xenopus, chick and mouse muscle development, growth and repair. We also study effects of environment such as training, exercise or aging.
METHODOLOGY:
Molecular developmental genetics and cell biology. Strong emphasis on development of in vivo imaging.
BIOGRAPHY:
Ph.D., 1980-1984, Cambridge University, Dr. Martin Brand - Visual transduction in retinal rod cells.
Postdoc, 1985-1987, University College London, Professor Martin Raff - Glial cell development in optic nerve.
Postdoc, 1987-1991, Stanford University, Professor Helen Blau - Mammalian muscle development.
Principle Investigator, 1992-2002, MRC Muscle and Cell Motility Unit and Developmental Biology Research Centre, King's College London.
Principle Investigator, 2002-date, MRC External Scientific Staff; Professor of Developmental Cell Biology, Randall Division for Cell and Molecular Biophysics, King's College London and member of MRC Centre for Developmental Neurobiology.
Non-Zebrafish Publications
*Anderson, C., *Williams, V.C., Moyon, B., Daubas, P., Tajbakhsh, S., Buckingham, M.E., Shiroishi, T., Hughes, S.M. and A-G. Borycki (2012) Sonic hedgehog acts cell-autonomously on muscle precursor cells to generate limb muscle diversity. Genes Dev. 26:2103-17. PMID: 22987640
Madhala-Levy, D., Williams, V.C., Hughes, S.M., Reshef, R. and O. Halevy (2012) Cooperation between Shh and IGF-I in Promoting Myogenic Proliferation and Differentiation via the MAPK/ERK and PI3K/Akt Pathways Requires Smo Activity. J. Cell. Physiol. 227:1455-64 PMID: 21618536
• *Henriquez, J-P.. *Webb, A., Bence, M., Bildsoe, H., Sahores, M., Hughes, S.M. and P.C. Salinas (2008) Wnt signalling promotes AChR aggregation at the neuromuscular synapse in collaboration with Agrin. Proc. Natl. Acad. Sci. USA. 105: 18812-18817. PMID: 19020093
• Maggs, A.M., Huxley, C. and S.M. Hughes (2008) Nerve-dependent changes in skeletal muscle myosin heavy chain after experimental denervation, cross-reinnervation and in a demyelinating mouse model of Charcot-Marie-Tooth disease type 1A. Muscle Nerve. 38: 1572–1584, PMID: 19016545
• *Chargé, S.B., *Brack, A.S.,Bayol, S.A. and S.M. Hughes (2008) MyoD- and nerve-dependent maintenance of MyoD expression in mature muscle fibres acts through the DRR/PRR element. BMC Devel. Biol. 8: 5. PMID: 18215268
• Lange, S., Xiang, F., Yakovenko, A., Vihola, A., Rostkova, E., Hackman, P., Kristensen, J., Brandmeier, B., Franzen, G., Hedberg, B. et al. (2005). The kinase domain of titin controls muscle gene expression and protein turnover. Science 308:1599-1603.
• Brack, A.S., Bildsoe, H. and S.M. Hughes. (2005) Evidence that satellite cell decrement contributes to preferential decline in nuclear number from large fibres during murine age-related muscle atrophy J. Cell Sci. 118:4813-4821.
• Bruusgaard, J.C., Brack, A.S., Hughes, S.M. and K. Gundersen (2005) Muscle hypertrophy induced by the Ski protein: cyto-architecture and ultrastructure. Acta Physiol. Scand. 185:141-149.
• *Schuierer, M.M., *Mann, C.J., Bildsoe, H., Huxley, C. and S.M. Hughes (2005) Analyses of the differentiation potential of satellite cells from myoD-/-, mdx, and PMP22 C22 mice. BMC Musculoskeletal Disord. 6:15.
• *Li, X., *Blagden, C.S., *Bildsoe, H., Bonnin, M-A., Duprez, D. and S.M. Hughes (2004) Hedgehog can drive terminal differentiation of amniote slow skeletal muscle. BMC Dev. Biol. 4: 9.
• Grimaldi, A., Tettamanti, G., Martin, B.,L. Gaffield, W., Pownall, M.E. and S.M. Hughes (2004) Hedgehog regulation of superficial slow muscle fibres in Xenopus and the evolution of tetrapod trunk myogenesis. Development. 131: 3249-3262.
• Jazwinska, A., Elher, E. and S.M. Hughes (2003) Intermediate filament-co-localized myosin heavy chain-like molecules define distinct cellular domains in hair follicles and epidermis (2003) BMC Cell Biology 4(1): 10.
• Krylova, O., Herreros, J. Cleverley, K., Ehler, E., Hughes, S.M. and P.C. Salinas. (2002) Wnt-3, expressed by motoneurons, regulates terminal arborization of neurotrophin-3-responsive spinal sensory neurons. Neuron 35: 1043-1056.
• Chargé, S.B., Brack, A.S. and S.M. Hughes (2002) Aging-related satellite cell differentiation defect occurs prematurely after Ski-induced skeletal muscle hypertrophy. Am. J. Physiol. 283: C1228-C1241.
• Lluis, F., Roma, J., Suelves, M., Parra, M., Aniorte, G., Gallardo, E., Illa, I., Rodriguez, L., Hughes, S.M., Carmeliet, P., Roig, M. and P. Munoz-Canoves (2001) Urokinase-dependent plasminogen activation is required for efficient skeletal muscle regeneration in vivo. Blood 97: (6) 1703-1711.
• Maggs, A.M., Taylor-Harris, P., Peckham, M. and S.M. Hughes (2000) Evidence for differential post-translational modifications of slow myosin heavy chain during murine skeletal muscle development. J. Muscle Res. Cell Motil. 21: (2) 101-113.
• Hook, P., Li, X., Sleep, J., Hughes, S.M. and L. Larsson (1999) Motility of slow myosin extracted from single muscle cells in young and old rat soleus muscle. J. Physiol. 520: 463-471.
• Hughes, S.M., Chi, M.M-Y., Lowry, O.H. and K. Gundersen (1999) Myogenin induces a shift in enzyme activity from glycolytic to oxidative metabolism in muscles of transgenic mice. J. Cell. Biol. 145(3):633-642.
• Robson, LG. and S.M. Hughes (1999) Local signals in the chick limb bud can override myoblast lineage commitment: induction of slow myosin hevay chain in fast myoblasts. Mech. Dev. 85: 59-71.
• Hughes, S.M. (1999) Fetal myoblast clones contribute to both fast and slow fibres in developing rat muscle. Int. J. Dev. Biol. 43:149-155.
• Hughes, S.M. and P.C. Salinas (1999) Control of muscle fibre and motoneuron diversification. Curr. Opin. Neurobiol. 9, 54-64.
• Blagden, C.S. and S.M. Hughes (1999) Extrinsic influences on limb muscle organisation. Cell Tissue Res. 296, 141-150.
• Hughes, S.M. (1998) Muscle development: electrical control of gene expression. Current Biology. 8, R892-R894.
• Hughes, S.M., C.S. Blagden, X. Li and A. Grimaldi. (1998) The role of Hedgehog proteins in vertebrate slow and fast skeletal muscle patterning. Acta Physiol. Scand. 163, S7-S10.
• Blake, J., Salinas, P.C. and Hughes, S.M. (1997) nßgeo, a combined selection and reporter gene for retroviral and transgenic studies. Biotechniques, 23: 690-695.
• Hughes, S.M., Koishi, K., Rudnicki, M. and Maggs, A.M. (1997) MyoD protein is differentially accumulation in fast and slow skeletal muscle and required for normal fibre type balance in rodents. Mech. Dev. 61: 151-163.
• Li, X., S.M. Hughes, G. Salviati, A. Teresi and L. Larsson (1996) Thyroid hormone effects on contractility and myosin composition of soleus muscle and single fibres from young and old rats. J. Physiol. 494: 555-567.
• Robson, L.G. and S.M. Hughes (1996) The distal limb environment regulates MyoD accumulation and muscle differentiation in mouse-chick chimaeric limbs. Development 122: 3389-3910.