Person
Gerster, Thomas
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Biography and Research Interest
*** Lab was affiliated with the Dept. of Cell Biology at the Biozentrum of the University of Basel, Switzerland from 1991 to 1999. No longer involved in zebrafish research. ***
If you require any of the plasmid probes generated in the lab please contact the provided current address.
If you require any of the plasmid probes generated in the lab please contact the provided current address.
Non-Zebrafish Publications
Hauptmann, G. & Gerster, T.'Multicolor whole-mount in situ hybridization to Drosophila embryos'
Dev. Genes Evol. 1996 Dec (206): 292-295
ABSTRACT
We report an extended whole-mount in situ hybridization procedure for Drosophila embryos. By using probes labelled with digoxigenin, fluorescein and biotin, respectively, this protocol allows the detection in three colors of RNAs derived from three different genes. Hybridized probes are detected by consecutive staining with appropriate alkaline phosphatase conjugates using different chromogenic substrate combinations, and serial removal of the antibody conjugates by low pH washes.
HAUPTMANN, G. & GERSTER, T. (2000) 'Multicolor Whole-Mount In Situ Hybridization' in: 'Developmental Biology Protocols vol. III' (eds. R.S. Tuan and C.W. Lo) Meth. Mol. Biol. 137, 139-148. (There is no abstract to this method paper.)