Morpholino

MO1-dhrs9

ID

ZDB-MRPHLNO-050926-1

Name

MO1-dhrs9

Previous Names

MO1-rdh1l
rdh1l GT-MO (1)

Target

dhrs9 (1)

Sequence

5' - TCTGTCAGTGACTCACCCTTCTGTC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

Splice blocking morpholino. Sequence verified by author. This morpholino matches accession CAAK01001941 (Ensembl version 4 scaffold Zv4_533), but has a one base mismatch to comparable sequence from Ensembl version 5 scaffold Zv5_1468.

Genome Resources

None

Target Location

Genomic Features

No data available

Expression

Gene expression in Wild Types + MO1-dhrs9

Expressed Gene	Anatomy	Figures
crestin	hindbrain neural crest cell midbrain neural crest cell neural crest cell pharyngeal arch neural crest	Fig. 4, Fig. 5 from Wang et al., 2013
crip2	cardiac neural crest	Fig. 5 from Wang et al., 2013
dlx2a	pharyngeal arch 1 neural crest cell pharyngeal arch 2 neural crest cell pharyngeal arch 3-7 neural crest cell	Fig. 5 from Wang et al., 2013
myh6	atrium	Fig. 2 from Wang et al., 2013
myh7	cardiac ventricle presumptive cardiac ventricle heart tube	Fig. 2 from Wang et al., 2013
myl7	heart	Fig. 3 from Wang et al., 2013
pitx2	diencephalon lateral plate mesoderm left side	Fig. 3 from Wang et al., 2013
ptgs2a	unspecified	Fig. 3 from Eisinger et al., 2006
snai1b	neural crest cell	Fig. 4 from Wang et al., 2013

Phenotype

Phenotype resulting from MO1-dhrs9

Phenotype	Fish	Figures
atrium decreased size, abnormal	AB + MO1-dhrs9	Fig. 2 from Wang et al., 2013
embryonic viscerocranium morphogenesis disrupted, abnormal	WT + MO1-dhrs9	text only from Nadauld et al., 2005
eye decreased size, abnormal	WT + MO1-dhrs9	text only from Nadauld et al., 2005
heart decreased contractility, abnormal	AB + MO1-dhrs9	Fig. 6 from Wang et al., 2013
heart decreased functionality, abnormal	AB + MO1-dhrs9	Fig. 6 from Wang et al., 2013
heart contraction decreased rate, abnormal	AB + MO1-dhrs9	Fig. 6 from Wang et al., 2013
heart looping arrested, abnormal	AB + MO1-dhrs9	Fig. 1 from Wang et al., 2013
heart looping disrupted, abnormal	AB + MO1-dhrs9	Fig. 3 from Wang et al., 2013
mandibular arch skeleton absent, abnormal	WT + MO1-dhrs9	text only from Nadauld et al., 2005
neural crest cell decreased amount, abnormal	AB + MO1-dhrs9	Fig. 4, Fig. 5 from Wang et al., 2013
neural crest cell migration process quality, abnormal	AB + MO1-dhrs9	Fig. 5 from Wang et al., 2013
pectoral fin absent, abnormal	WT + MO1-dhrs9	text only from Nadauld et al., 2005
pectoral fin development disrupted, abnormal	WT + MO1-dhrs9	text only from Nadauld et al., 2005
post-vent region curved ventral, abnormal	AB + MO1-dhrs9	Fig. 1 from Wang et al., 2013
whole organism decreased size, abnormal	AB + MO1-dhrs9	Fig. 1 from Wang et al., 2013

Phenotype of all Fish created by or utilizing MO1-dhrs9

Phenotype	Fish	Conditions	Figures
neural crest cell decreased amount, abnormal	AB + MO1-dhrs9	standard conditions	Fig. 4, Fig. 5 from Wang et al., 2013
neural crest cell migration process quality, abnormal	AB + MO1-dhrs9	standard conditions	Fig. 5 from Wang et al., 2013
post-vent region curved ventral, abnormal	AB + MO1-dhrs9	standard conditions	Fig. 1 from Wang et al., 2013
heart decreased contractility, abnormal	AB + MO1-dhrs9	standard conditions	Fig. 6 from Wang et al., 2013
heart decreased functionality, abnormal	AB + MO1-dhrs9	standard conditions	Fig. 6 from Wang et al., 2013
atrium decreased size, abnormal	AB + MO1-dhrs9	standard conditions	Fig. 2 from Wang et al., 2013
heart looping disrupted, abnormal	AB + MO1-dhrs9	standard conditions	Fig. 3 from Wang et al., 2013
heart contraction decreased rate, abnormal	AB + MO1-dhrs9	standard conditions	Fig. 6 from Wang et al., 2013
whole organism decreased size, abnormal	AB + MO1-dhrs9	standard conditions	Fig. 1 from Wang et al., 2013
heart looping arrested, abnormal	AB + MO1-dhrs9	standard conditions	Fig. 1 from Wang et al., 2013
embryonic viscerocranium morphogenesis disrupted, abnormal	WT + MO1-dhrs9	standard conditions	text only from Nadauld et al., 2005
mandibular arch skeleton absent, abnormal	WT + MO1-dhrs9	standard conditions	text only from Nadauld et al., 2005
pectoral fin development disrupted, abnormal	WT + MO1-dhrs9	standard conditions	text only from Nadauld et al., 2005
pectoral fin absent, abnormal	WT + MO1-dhrs9	standard conditions	text only from Nadauld et al., 2005
eye decreased size, abnormal	WT + MO1-dhrs9	standard conditions	text only from Nadauld et al., 2005

Citations