Morpholino

MO1-gdf6a

ID

ZDB-MRPHLNO-050708-1

Name

MO1-gdf6a

Previous Names

rdr gtMO (1)
rdr^gtMO (1)

Target

gdf6a (1)

Sequence

5' - GCAATACAAACCTTTTCCCTTGTCC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

Splice-blocking morpholino, resulting in production of gdf6a mRNA containing its lone intron. This allows for normal maternal gdf6a function, as gdf6a is required for patterning the early embryo, but inhibits zygotic gdf6a function prior to eye development. As high levels of necrosis are observed in gdf6a morphants, gdf6a morpholinos were co-injected with a p53 translation blocking morpholino. -- French et al. 2007, ZDB-PUB-070726-17

Genome Resources

None

Target Location

Genomic Features

No data available

Expression

Gene expression in Wild Types + MO1-gdf6a

Expressed Gene	Anatomy	Figures
en1a	fast muscle cell muscle pioneer somite unspecified	Fig. 4 from Kawakami et al., 2005
nkx2.2a	floor plate	Fig. 4 from Kawakami et al., 2005
sox10	neural crest	Fig. 1 from Reichert et al., 2013

Phenotype

Phenotype resulting from MO1-gdf6a

Phenotype	Fish	Figures
cranial nerve II hypoplastic, abnormal	AB + MO1-gdf6a + MO4-tp53	Fig. 4 from Asai-Coakwell et al., 2007
eye decreased size, abnormal	AB + MO1-gdf6a + MO4-tp53	Fig. 3 from Asai-Coakwell et al., 2009 Fig. 4, Fig. 5 from Asai-Coakwell et al., 2007
eye morphology, abnormal	ua1013Tg + MO1-gdf6a	Fig. 4 from Hocking et al., 2018
lens protruding, abnormal	AB + MO1-gdf6a + MO4-tp53	Fig. 4, Fig. 5 from Asai-Coakwell et al., 2007
lens vacuolated, abnormal	AB + MO1-gdf6a + MO4-tp53	Fig. 4 from Asai-Coakwell et al., 2007
muscle pioneer increased amount, abnormal	WT + MO1-gdf6a + MO4-tp53	Fig. 7 from Nguyen-Chi et al., 2012
optic fissure closure incomplete, abnormal	AB + MO1-gdf6a + MO4-tp53	Fig. 3 from Asai-Coakwell et al., 2009 Fig. 4, Fig. 5 from Asai-Coakwell et al., 2007
post-vent region kinked, abnormal	AB + MO1-gdf6a + MO4-tp53	Fig. 3 from Asai-Coakwell et al., 2009
retina disorganized, abnormal	AB + MO1-gdf6a + MO4-tp53	Fig. 4 from Asai-Coakwell et al., 2007
retina malformed, abnormal	AB + MO1-gdf6a + MO4-tp53	Fig. 5 from Asai-Coakwell et al., 2007
retina morphology, abnormal	WT + MO1-gdf6a + MO4-tp53	Fig. 7 from French et al., 2007
superior ocular sulcus closure incomplete, abnormal	ua1013Tg + MO1-gdf6a	Fig. 4 from Hocking et al., 2018
superior ocular sulcus morphology, abnormal	ua1013Tg + MO1-gdf6a	Fig. 4 from Hocking et al., 2018
trunk vasculature broken, abnormal	WT + MO1-gdf6a	Fig. 1 from Krispin et al., 2017

Phenotype of all Fish created by or utilizing MO1-gdf6a

Phenotype	Fish	Conditions	Figures
eye decreased size, abnormal	AB + MO1-gdf6a + MO4-tp53	standard conditions	Fig. 3 from Asai-Coakwell et al., 2009 Fig. 4, Fig. 5 from Asai-Coakwell et al., 2007
cranial nerve II hypoplastic, abnormal	AB + MO1-gdf6a + MO4-tp53	standard conditions	Fig. 4 from Asai-Coakwell et al., 2007
post-vent region kinked, abnormal	AB + MO1-gdf6a + MO4-tp53	standard conditions	Fig. 3 from Asai-Coakwell et al., 2009
lens vacuolated, abnormal	AB + MO1-gdf6a + MO4-tp53	standard conditions	Fig. 4 from Asai-Coakwell et al., 2007
lens protruding, abnormal	AB + MO1-gdf6a + MO4-tp53	standard conditions	Fig. 4, Fig. 5 from Asai-Coakwell et al., 2007
retina disorganized, abnormal	AB + MO1-gdf6a + MO4-tp53	standard conditions	Fig. 4 from Asai-Coakwell et al., 2007
optic fissure closure incomplete, abnormal	AB + MO1-gdf6a + MO4-tp53	standard conditions	Fig. 3 from Asai-Coakwell et al., 2009 Fig. 4, Fig. 5 from Asai-Coakwell et al., 2007
retina malformed, abnormal	AB + MO1-gdf6a + MO4-tp53	standard conditions	Fig. 5 from Asai-Coakwell et al., 2007
muscle pioneer increased amount, abnormal	WT + MO1-gdf6a	chemical treatment: SU5402	Fig. 7 from Nguyen-Chi et al., 2012
trunk vasculature broken, abnormal	WT + MO1-gdf6a	standard conditions	Fig. 1 from Krispin et al., 2017
muscle pioneer increased amount, abnormal	WT + MO1-gdf6a	standard conditions	Fig. 7 from Nguyen-Chi et al., 2012
retina morphology, abnormal	WT + MO1-gdf6a + MO4-tp53	standard conditions	Fig. 7 from French et al., 2007
muscle pioneer increased amount, abnormal	WT + MO1-gdf6a + MO4-tp53	standard conditions	Fig. 7 from Nguyen-Chi et al., 2012
eye morphology, abnormal	ua1013Tg + MO1-gdf6a	standard conditions	Fig. 4 from Hocking et al., 2018
superior ocular sulcus morphology, abnormal	ua1013Tg + MO1-gdf6a	standard conditions	Fig. 4 from Hocking et al., 2018
superior ocular sulcus closure incomplete, abnormal	ua1013Tg + MO1-gdf6a	standard conditions	Fig. 4 from Hocking et al., 2018

Citations