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Fig. 4 CXCR3 (CXC motif chemokine receptor 3) modulates endothelial cell (EC) motility and cellular alignment in response to a pulsatile flow regimen. A, Representative images of EC alignment to pulsatile flow at 0 and 24 hours, using ECs transfected with control (siControl) or siRNA against CXCR3 (siCXCR3). Scale bar=100 µm. B, Distribution angles of the siControl (gray) or siCXCR3 (magenta) EC?s longest axis relative to the direction of flow at 0 and 24 hours. C and D, Analysis of siControl or siCXCR3 motility under pulsatile flow for 24 hours. C, Representative cell tracks where arrowheads represent the direction of cellular movement and circles represent the starting point of individual cells. D, Quantification of the total distance of movement from the cells? origin. E, Quantification of the length/width aspect ratio of siControl or siCXCR3 ECs under pulsatile flow at 0 and 24 hours. For D (n=71) and E (n=180), each dot represents an individual cell, pooled from 3 individual experiments. Statistical analyses were performed using the Mann-Whitney U test (D) and Kruskal-Wallis test with Dunn multiple comparisons test (E; omnibus P<0.0001). Data are presented as box plots that display the median value with first and third quartiles and min/max bars. All portions represent data from at least 3 independent experiments. *P<0.05, ****P<0.0001.