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Fig. 3 EAE predisposes to changes in adult atrial and ventricular chamber shape and size. A. Schematic of embryonic alcohol treatment, embryo sorting, and adult heart evaluations. B?G. Fluorescent images of dissected CTRL and EAE Tg(myl7:nGFP) hearts at 8 mpf showing atrial enlargement and reduced ventricle size in 8-month adults. a = atrium, v = ventricle, and oft = outflow tract. H. Ventricle and atrial area normalized to body length and atrial:ventricular area (%) in 8 mpf males (n > 12). I. Ventricle and atrial area normalized to body length and atrial:ventricular area (%) in 8 mpf females (n > 13). J. Cardiomyopathy incidence in dissected male and female 8 mpf hearts (Fisher's exact test, n > 11). For H?I, statistical significance was determined using an unpaired, two-tailed Student's t-test when comparing normally distributed samples or a nonparametric Mann?Whitney test for those without a normal distribution. P-values are shown. Scale bars: 100 ?m. ns, non-significant (P > 0.05).