Fig. 4

Fig. 4 Cdh5 clusters detach from junctions and move toward the apical compartments in rasip1 mutants.

A–C Time-lapse images of Cdh5-Venus in wild-type embryos (A) and in rasip1^ubs28 mutants (B, C). Magenta arrowheads mark the dots, clusters, or linear structures of Cdh5 within the apical compartments. Green arrowheads mark the newly formed junctions at the boundaries after the detachment of old junctions. (VI) Arrow maps show the speed and direction of the flow of Cdh5 between the two time points in (V). The arrows are twice the length of the actual movement for visualization purposes, with their colors representing velocities relative to the centers of the apical compartments. Consistent observations were made in over 20 samples from 6 independent experiments per group. D, E Heat maps showing the average velocity of Cdh5 in the radial direction at 6-minute interval in wild-type embryos (D) and rasip1^ubs28 mutants (E). Average velocity maps were generated from frames with a 36-second interval using PIV and were further combined into a heat map displaying the total particle movement over 360 seconds in the radial direction. Colors in the heat map represent the velocities of movement relative to the centers of the apical compartments. Black arrowheads mark outward movement, while black arrows mark inward movement. F Average radial velocity of all Cdh5 in (D) and (E), measured over 2-minute intervals. G–I Diagrams (G) and time-lapse images of photo-conversion on the junctional clusters in the apical compartments of wild-type embryos (H) and rasip1 mutants (I). White arrowheads mark the converted Cdh5-mClav that moved to the boundaries. All scale bars: 5 μm. Source data are provided as a Source Data file.