Fig. 3

Fig. 3 Cdh5 is poorly restricted to the junctions in rasip1 mutants.

A Schematic drawing of the photo-conversion of mClavGR2 and cdh5-mClav cDNA recombined into the cdh5 BAC clone. B Diagrams of photo-conversions at the initial cell-cell contact sites (anastomotic patches) in wild-type embryos and in rasip1 mutants. C, D Time-lapse imaging of Cdh5-mClav after photo-conversion specifically at junctional patches. White arrowheads mark the converted Cdh5-mClav. White arrows indicate the junctional ring and reticulated junctions formed in wild-type embryos and rasip1 mutants, respectively. Consistent observations were made in over 6 samples from 3 independent experiments per group. E–H Photo-conversions on half junctional rings as depicted in diagrams for wild-type embryos (E) and equivalent regions in rasip1 mutants (G). (E’, G’) Time-lapse imaging of Cdh5-mClav after photo-conversion of half junctional rings in wild-type embryos (E’) and equivalent regions in rasip1 mutants (G’). White dashed lines mark the converted region before conversion. Blue and orange arrowheads indicate the unconverted and converted half rings, respectively. White arrows indicate converted Cdh5 in the apical domain. (E”,G”) Intensities of converted and unconverted Cdh5-mClav along the white lines in (E’) and (G’), corresponding to the red dashed lines in the diagrams in (E) and (G). F The relative intensity of green Cdh5-mClav in the converted half rings compared to the unconverted half rings in wild-type embryos (n = 5 experiments) and rasip1 mutants (n = 6 experiments), presented as mean ± SD. N.s., not significant, 2-tailed unpaired t-test on the area under the curve. H The relative intensity of magenta Cdh5-mClav in the unconverted half rings compared to the converted half rings in wild-type embryos (n = 5 experiments) and rasip1 mutants (n = 6 experiments), presented as mean ± SD. **P = 0.0028, 2-tailed unpaired t-test on the area under the curve. All scale bars: 5 μm. Source data are provided as a Source Data file.