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Fig. 7 Immature ovaries and depleted gonadotrophs in lhx4 KO females. (A) Hematoxylin and eosin histology performed on adult fish ovary sections, demonstrating an undeveloped ovary of homozygous lhx4-mutant female (top), compared with a properly developed WT sibling ovary (bottom). Scale bar = 200 µm. (B) Expression of RFP under the tilapia lhb promoter in the pituitary of adult WT sibling female (left) and homozygous lhx4-mutant female (right), indicating severely impaired gonadotroph development in the mutant. Scale bar = 100 µm.
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Full text @ Int. J. Mol. Sci.