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Figure 5 trib manipulation does not affect leukocyte number in zebrafish larvae. (A) Example brightfield and fluorescent micrographs of neutrophils (Tg(mpx:GFP)i114) in four 2 dpf larvae injected at the one-cell stage with either phenol red (PR), trib1 RNA overexpression (OE), trib1 CRISPant, trib3 RNA overexpression (OE), or trib3 CRISPant. Scale bars = 200 µM. (B) Example brightfield and fluorescent micrographs of macrophages (Tg(mpeg:nlsclover)sh436) in four 2 dpf larvae injected at the one-cell stage with either PR, trib1 RNA overexpression (OE), trib1 CRISPant, trib3 RNA overexpression (OE), or trib3 CRISPant. Scale bars = 200 µM. (C) Wholebody neutrophil numbers (Tg(mpx:GFP)i114) in 2 dpf larvae injected at the one-cell stage with either PR, trib1 RNA overexpression (T1 OE), trib1 CRISPant (T1 cpr), trib3 RNA overexpression (T3 OE), or trib3 CRISPant (T3 cpr). Data shown are mean ± standard error of the mean (SEM), n = 30 fish per group. (D) Wholebody macrophage numbers (Tg(mpeg:nlsclover)sh436) in 2 dpf larvae injected at the one-cell stage with either PR, trib1 RNA overexpression (T1 OE), trib1 CRISPant (T1 cpr), trib3 RNA overexpression (T3 OE), or trib3 CRISPant (T3 cpr). Data shown are mean ± SEM, n = 30 fish per group. (E) Total tail neutrophil numbers (Tg(mpx:GFP)i114) in 3 dpf larvae injected at the one-cell stage with either PR or trib1 RNA overexpression (T1 OE). Data shown are mean ± SEM, n = 30 fish per group. (F) Total tail neutrophil numbers (Tg(mpx:GFP)i114) in 3 dpf larvae injected at the one-cell stage with either tyrosinase control CRISPant (tyr cpr) or trib1 CRISPant (T1 cpr). Data shown are mean ± SEM, n = 30 fish per group. (G) Total tail neutrophil numbers (Tg(lyz: nfsB.mCherry)sh260) in 2 and 3 dpf larvae injected at the one-cell stage with either PR or trib1 RNA overexpression (T1 OE). Data shown are mean ± SEM, n = 37–42 fish per group. (H) Total tail neutrophil numbers (Tg(lyz: nfsB.mCherry)sh260) in 2 and 3 dpf larvae injected at the one-cell stage with either tyrosinase control CRISPant (tyr cpr) or trib1 CRISPant (T1 cpr). Data shown are mean ± SEM, n = 60 fish per group.