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Fig. 6 Effect of Stat3 mutations on the response to G-CSF. Wild-type (WT: a, d), knockout (KO: b, e) and transactivation domain truncation (ΔTAD: c, f) mutant 1 cell embryos on the Tg(mpx:GFP) transgenic background were left uninjected (a–c) or subjected to injection with mRNA encoding the zebrafish G-CSF paralogue, Csf3a, and imaged 3 days post injection showing representative images with scale bars of 100 μm. Quantitation of mpx+ cells (g) showing values for individual embryos. Gene expression analysis of the indicated genes in Csf3a-injected WT, KO and ΔTAD embryos (n = 6) presented as fold-change (log2) relative to WT (h). g, h show mean and SEM with statistical significance indicated between genotypes (g) or compared to WT (h) (***p < 0.001, **p < 0.01, ns: not significant) or between uninjected and injected (###p < 0.01, ##p < 0.01, #p < 0.05).