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Fig. 4 Effect of Stat3 mutations on the response of neutrophils and macrophages to injury. Wild-type (WT), knockout (KO), and transactivation domain truncation (ΔTAD) mutant embryos on either the Tg(mpx:GFP) or Tg(mpeg1.1:GFP) transgenic background were subjected to a caudal fin wounding assay at 3 dpf and imaged using fluorescence microscopy from 0 to 24 h post wounding (hpw) (a). Representative images of mpx+ neutrophil (b) and mpeg1.1+ macrophage (e) cells migrating to the vicinity of the injury site (dotted line) at the indicated times, with scale bars of 100 μm. Quantitation of total migrating mpx+ cells (c) or normalized relative to overall mpx+ cells (d), as well as total migrating mpeg1.1+ cells (f) or normalized relative to overall mpeg1.1+ cells (g) (n = 5). Gene expression analysis of the indicated genes in WT, KO, and ΔTAD embryos (n = 5–6) at 4 hpw presented as fold change (log2) relative to WT (h). c, d, f-h show mean and SEM with statistical significance indicated compared to WT (***p < 0.001, **p < 0.01, *p < 0.05), between mutants (@@@p < 0.001, @@p < 0.01, @p < 0.05) or between unwounded and wounded (###p < 0.001, ##p < 0.01, #p < 0.05), identified using a two-way ANOVA with Tukey’s multiple comparison test.