ZFIN Image: Fabian et al., 2024, Figure 5

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Figure Caption

Figure 5

Muscle and sarcomere organization is defective in neb mutants. (A) Confocal micrographs of wholemount preparations of embryos at 6 dpf stained with antibody against α-Actinin and Rhodamine Phalloidin. Nonsense mutants and the splice mutant neb²¹ show disorganized, misaligned or broken muscle fibers (arrows), defects in the MTJ regions, with partially detached fibers and accumulations of aggregates of actin filaments (arrowheads), and aggregates of actin/α-Actinin (open arrowheads). Scale bar = 20 μm. (B) Transmission electron micrographs of zebrafish embryos at 6 dpf showing defects in the MTJ regions (dotted line), with disorganized muscle fibers (arrows) and misaligned sarcomeres (arrowheads) in the nonsense, neb³⁴, and neb²¹ splice mutants. Scale bars = 2 μm.

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Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Hum. Mol. Genet.