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Fig. 5 Hes1 plays a crucial role in neural induction by acting as a suppressor of Wnt3 gene expression. (A, B) Western Blot was used to analyse the interference efficiency of Hes1. Full-length gels before cropping are noted in Fig. S8. (C,D) Western Blot was used to detect the expression of Wnt3 after Hes1 knockdown. Full-length gels before cropping are noted in Fig. S8. (E,F) Following the knockdown of Hes1, qPCR was utilized to evaluate the expression of Sox2, Pax6 and Oct4, Nanog. (G,H) Western Blot was used to detect the expression of Sox2 and Oct4 after Hes1 knockdown. Full-length gels before cropping are noted in Fig. S8. Data are presented as means ± SEM of at least three independent experiments (B,D,E,F,H), ** (p < 0.01), *** (p < 0.001).