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Fig. 2 (A) The prps1a expression in the otic vesicle of zebrafish. In situ hybridization whole mounts of 1-dpf zebrafish (wild-type AB) with the prps1a antisense (left) and sense (right) mRNA probes. With the prps1a antisense probe, specific moderate expression was observed in the entire embryo body, with more intense signals seen in the eyes, brain, gut, and otic vesicles, compared with the sense probe stain. The otic vesicles of the embryos are marked by dots. (B) Saccular epithelium (SE), lagenar epithelium (LE), and utricular epithelium (UE) of otolith organs of Et(krt4:GFP) sqet4 adult zebrafish. Green dots in the epithelia are hair cell bodies expressing GFP. D, dorsal; M, medial; and P, posterior. Scale bar = 100 μm. (C) DNA gels of RT-PCR products from saccular, utricular, and lagenar epithelia of the Et(krt4:GFP) sqet4 adult zebrafish, showing the prps1a and prps1b expression in all three sensory epithelia of otolith organs. +: with reverse transcriptase, and –: without reverse transcriptase. ß-actin was used as a positive control.